浣滆? Michael Kaszuba and Pauline Carnell, 椹皵鏂囦華鍣ㄥ叕鍙?
涓烘垚鍔熷疄鐜拌嵂鐗╀紶杈擄紝鐞嗘兂鐨勯斿緞鏄皢娌葷枟鍒跺墏闈跺悜浼犺緭鑷蟲墍闇浣嶇疆錛屽疄鐜板鍙楄嵂緇勭粐涓庤嵂鐗╅棿鐩鎬簰浣滅敤鐨勭洃嫻嬨傚彲騫挎硾榪愮敤浜庡叏韜強灞閮ㄧ粰鑽殑鑴傝川浣撳榪欎竴搴旂敤鐨勯渶姹備笉鏂笂鍗囥傜敱浜庡叿澶囨恫浣撱佸浐浣撱佸崐鍥轟綋閰嶆柟鎼鴻澆鑳藉姏錛岃剛璐ㄤ綋宸插簲鐢ㄤ簬閽堝鐪熻弻鎰熸煋銆佺敳鑲濄佹ユф穻宸寸粏鑳炵櫧琛鐥呯瓑鐤劇梾鐨勬不鐤楀疄璺典腑銆傝剛璐ㄤ綋鐨勭墿鐞嗚〃寰佸閰嶆柟鏀硅壇鍙婄爺鍙戝叿鏈夊崄鍒嗛噸瑕佺殑鎰忎箟銆傛湰鏂囧皢鎺㈣涓ら」鐩稿叧杈呭姪鎶鏈殑鎴愬姛搴旂敤錛氫竴鏄敤浜庢嫻嬭剛璐ㄤ綋綺掑害鍒嗘瀽鐨勭撼綾崇矑瀛愯窡韙垎鏋愭妧鏈紙NTA錛夛紝浜屾槸鐢ㄤ簬嫻嬮噺鑴傝川浣撶矑搴﹀強zeta鐢典綅鐨勫厜鏁ｅ皠錛堝姩鎬佺數娉籌級鎶鏈?
鑳藉鈥滃畾浣嶁濈殑緇欒嵂杞戒綋
鑴傝川浣撶敱鑴傝川鍙屽垎瀛愬眰鏋勬垚錛屾槸涓縐嶄漢宸ュ埗澶囩殑鐞冨艦鍥婃場銆傛渶鍒濆簲鐢ㄤ簬鐮旂┒鑶滅粨鏋勬笚閫忔х殑妯″瀷緋葷粺涓傜敱浜庤剛璐ㄤ綋鏃㈣兘灝嗘按婧舵х墿璐ㄥ寘灝佷簬鍏跺泭娉′腑錛屽張鑳藉皢娌規憾鎬х墿璐ㄥ寘灝佷簬鍏惰剛鍙屽眰鍐咃紝榪戝勾鏉ュ瀹冪殑鐮旂┒涓昏闆嗕腑鍦ㄥ叾浣滀負緇欒嵂杞戒綋鐨勯傜敤鎬с傞氳繃鎺у埗鍏惰剛璐ㄦ瀯鎴愬茍/鎴栬呰繘琛岃〃闈㈡敼鎬э紝鐮旂┒浜哄憳榪樿兘澶熼拡瀵瑰叿浣撶殑搴旂敤瀵硅剛璐ㄤ綋榪涜璁捐銆傝剛璐ㄤ綋鍒嗕負鍗曞鑴傝川浣撳拰澶氬鑴傝川浣擄紝鍏鋒湁鐢熺墿闄嶈В鎬э紝鍩烘湰鏃犳瘨鎬э紝騫跺彲浠ュ埗鎴愪笉鍚岀矑搴︺傛棦鑳借澆鑽蜂翰姘寸墿璐紝涔熻兘杞借嵎鐤忔按鐗╄川鐨勭壒鎬т嬌鑴傝川浣撳叿鏈夊箍娉涚殑搴旂敤棰嗗煙銆傛澶栵紝渚嬪閫氳繃娣誨姞鎶椾綋鎴栬偨閾劇瓑鐗╄川榪涜琛ㄩ潰鏀規э紝鑴傝川浣撹兘浣胯嵂鐗╅澏鍚戣揪鍒頒綋鍐呮寚瀹氬尯鍩燂紝鎴栬呭歡闀夸綋鍐呰嵂鏁堢殑浣滅敤鏃墮棿銆傝繖浜涚壒鎬т績浣胯澶氱爺絀朵漢鍛橀夋嫨鑴傝川浣撲綔涓鴻嵂鐗╄澆浣撱?
鑴傝川浣撳簲鐢ㄥ箍娉涳紝鍏跺湪鍒惰嵂棰嗗煙鐨勫簲鐢ㄥ寘鎷細淇濇姢娌葷枟鑽墏涓嶅彈鑳冭偁鐜褰卞搷錛屼績榪涜嵂鐗╂垚鍒嗗湪鑳冭偁閬撳唴鐨勪紶杈擄紝瀹氬悜鎺ч噴鑽墿鑷崇壒瀹氫綅緗紝騫惰兘淇冭繘婧惰В浠ュ強澧炲姞緇嗚優瀵硅嵂鐗╃殑鍚告敹銆備笌姝ゅ悓鏃訛紝鑴傝川浣撳埗鍓傜殑灞閮ㄥ鐢ㄥ姛鑳戒篃寮曡搗浜嗗尰鑽強鍖栧鍝佽涓氱殑寮虹儓鍏蟲敞銆傝剛璐ㄤ綋瀹炵幇鑽墿寰矑闈跺悜浼犺緭鐨勬満鐞嗗寘鎷墿鏁ｅ拰鐩存帴緇嗚優铻嶅悎錛屽叾涓悗鑰呮洿涓哄父瑙併傚湪鐩存帴緇嗚優铻嶅悎涓紝鑴傝川浣撶殑鑴傚弻灞傚彲涓庡叾浠栧弻鍒嗗瓙灞傦紙濡傜粏鑳炶啘錛夎瀺鍚堬紝浠庤屽皢鑴傝川浣撶殑鍐呭惈鐗╀紶杈撹嚦鐩爣緇嗚優銆?
鐗╃悊琛ㄥ緛浣滅敤
鑴傝川浣撶殑鏁堢敤涓庡叾鐗╃悊鐗規у瘑鍒囩浉鍏熾傛祴閲忓拰鍒嗘瀽鑴傝川浣撳埗鍓傜殑綺掑害銆佹祿搴︺亃eta鐢典綅絳夊弬鏁版湁鍔╀簬棰勪及鍒跺墏鍦ㄤ復搴婄幆澧冧笅鐨勬晥鐢ㄣ傚湪榪欎簺鍙傛暟涓紝鑴傝川浣撶殑綺掑害鍙婂垎甯冨挨涓洪噸瑕侊紝灝ゅ叾鏄湪鑲洪儴鎴栭潪鑲犻亾閫斿緞緇欒嵂鐨勬儏鍐典笅錛岀矑搴﹀鑽墿媧繪ф垚鍒嗙殑鏈夋晥浼犺緭璧峰埌浜嗗叧閿綔鐢ㄣ傞櫎浣撳唴浣滅敤澶栵紝鑴傝川浣撶殑綺掑害榪樺叿鏈変竴緋誨垪浣撳鎰忎箟錛屼細瀵圭粰鑽郴緇熺殑杞借嵂鑳藉姏銆佽仛闆嗙▼搴﹀拰娌夌Н鐘跺喌絳夎澶氭柟闈駭鐢熷獎鍝嶃傝屽彟涓涓噸瑕佺殑鎸囨爣鏄痾eta鐢典綅鐨勬暟鍊兼祴閲忥紝璇ユ祴閲忔妧鏈凡騫挎硾搴旂敤浜庤兌浣撶郴緇熺殑紼沖畾鎬ч嫻嬨傚湪搴旂敤浜庤剛璐ㄤ綋鏃訛紝榪欎竴鎶鏈兘澶熺洃嫻嬩綋鍐呭彲鑳藉彂鐢熺殑甯︾數鑴傝川浣撲笌鐩稿弽鐢墊у井綺掗棿鐨勭浉浜掍綔鐢ㄣ?
鎻愰珮鍒嗘瀽鍙潬鎬?/strong>
灝卞尰鑽厤鏂圭爺鍙戣岃█錛屾棤璁洪噰鐢ㄤ綍縐嶈澆浣撱佷綍縐嶉厤娉曪紝蹇呴』瑕佹湁鍙潬鐨勫垎鏋愪綋緋繪彁渚涚浉鍏崇殑鍋ュ叏鏁版嵁銆傚鏋滅敤鑴傝川浣撲綔涓鴻澆浣擄紝涓哄疄鐜版洿楂樻晥銆佹洿鍏堣繘鐨勭爺鍙戣繘紼嬶紝闇鍦ㄨ繘琛屼換浣曚復搴婅瘯楠屼箣鍓嶏紝璇﹀敖浜嗚В榪欎簺杞戒綋鏄浣曞湪浣撳唴鍙戠敓浣滅敤鐨勶紝鑰岃繖鍏朵腑鐨勫叧閿究鏄矑搴﹀強zeta鐢典綅鐨勬祴閲忋?
浣跨敤綰崇背棰楃矑璺熻釜鍒嗘瀽鎶鏈祴閲忚剛璐ㄤ綋綺掑害
綰崇背棰楃矑璺熻釜鍒嗘瀽鎶鏈紙綆縐幫細NTA錛夎繍鐢ㄥ厜鏁ｅ皠鏉ユ嫻嬫憾娑蹭腑鐨勭撼綾抽綺掋傝鎶鏈粨鍚堜簡鍏夋暎灝勬樉寰暅鍙婅棰戞垚鍍忔妧鏈紙鍥句竴錛夛紝鏍規嵁涓嶅悓鐨勬牱鏈被鍨嬶紝鍙祴閲?0 -2000 nm鑼冨洿鍐呯殑綺掑害銆?
浣跨敤縐戝鏁扮爜鎽勭浉鏈哄彲浠ユ崟鎹夋憾娑蹭腑棰楃矑鐨勬暎灝勫厜錛屽啀鐢變華鍣ㄨ蔣浠墮愬撫榪借釜姣忎釜棰楃矑鐨勭Щ鍔ㄦ儏鍐碉紙鍥句簩錛夈?
鍦ㄥ琚収灝勬牱鏈繘琛屽獎鍍忚褰曞悗錛孨TA杞歡灝嗚瘑鍒茍璺熻釜瑙嗛噹涓瘡涓涓綺掔殑甯冩湕榪愬姩銆傛暟浣嶆崟鎹夊埌鐨勫崟涓矑瀛愮殑鎵╂暎閫熺巼錛堥熷害錛変笌鐞冧綋絳夋晥嫻佷綋鍔ㄥ姏鐩村緞鐩稿叧錛屽茍鑳介氳繃浠ュ竷鏈楄繍鍔ㄤ負妯″瀷鐨勬柉鎵樺厠鏂?鐖卞洜鏂潶鏂圭▼璁＄畻寰楀嚭銆侼TA鍙愮矑璁＄畻綺掑害錛屼笖鐢變簬鏈夊獎鍍忕墖孌靛仛鍒嗘瀽鍩虹錛岀敤鎴瘋繕鍙互綺劇‘琛ㄥ緛瀹炴椂鐘舵併備釜浣撻綺掔殑綺掑害鍒嗗竷閮借兘鍦ㄦ暟縐掑唴蹇熻幏寰椼?
NTA鎶鏈篃鑳藉瀵逛笉鍚岀殑鑴傝川浣撹繘琛屽悓姝ョ殑鍗曠嫭鍒嗘瀽錛屽洜姝ら櫎鍩虹鐨勭矑搴﹀垎鏋愪互澶栵紝榪樹負鑾峰彇棰濆淇℃伅鎻愪緵浜嗘柊鐨勯斿緞錛屽叾涓寘鎷崟涓剛璐ㄤ綋鐨勭浉瀵瑰厜鏁ｅ皠寮哄害鐨勬祴瀹氥傚皢鏁版嵁緇撴灉騫朵笌鍙﹁嫻嬪緱鐨勭矑搴︽暟鎹粯鎴愬潗鏍囧浘錛?nbsp;鍙互鏇村姞緇嗚嚧鍦板垎杈ㄥ嚭鐢變笉鍚屾姌灝勭巼錛圧I錛夋垨涓嶅悓鏉愭枡鏋勬垚鐨勯綺掋傝繖縐嶆柟娉曞紜畾鑴傝川浣撶殑鍐呭惈鐗╂槸鍚﹀彉寮傞潪甯告湁鐢紝鍥犱負絀哄泭娉＄殑RI鍊煎彲鑳戒綆浜庤澆鑽風殑鍥婃場銆傚悓鏃朵綔涓轟竴縐嶅崟綺掑瓙鎺㈡祴緋葷粺錛孨TA榪樿兘嫻嬮噺棰楃矑嫻撳害銆?
zeta鐢典綅嫻嬮噺鐨勫簲鐢?/strong>
鑴傝川浣撲笌緇嗚優鍦ㄤ綋鍐呭彂鐢熶氦浜掍綔鐢ㄧ殑閮ㄤ綅鍦ㄥ緢澶х▼搴︿笂鐢辮剛璐ㄤ綋鐨勭矑搴﹀喅瀹氥傚皬綺掑害鐨勮剛璐ㄤ綋鑳藉閫氳繃鑲濈鐘墮殭鐨勮啘瀛旓紝涓矑搴︾殑鑴傝川浣撳仠鐣欏湪琛娑茶厰瀹や腑騫惰兘闀挎椂闂村驚鐜祦鍔紝鑰屽ぇ綺掑害鐨勮剛璐ㄤ綋鍒欒Kupffer緇嗚優蹇熸竻闄ゃ備笌綺掑害嫻嬮噺綾諱技錛屼簡瑙ｈ剛璐ㄤ綋鍒跺墏鐨剒eta鐢典綅鏈夊姪浜庨嫻嬪叾鍦ㄤ漢浣撳唴鐨勫彉鍖栥?
棰楃矑鐨剒eta鐢典綅鏄寚棰楃矑鍦ㄧ壒瀹氬獟浠嬩腑鑾峰緱鐨勬葷數鑽楓備互鍩哄洜娌葷枟涓轟緥錛寊eta鐢典綅鐨勬祴閲忓彲鐢ㄤ簬浼樺寲鐗瑰畾鑴傝川浣撲笌涓嶅悓DNA璐ㄧ矑鐨勬瘮渚嬶紝浠庤屽皢閰嶆柟鐨勮仛闆嗗害闄嶅埌鏈浣庛?
鍔ㄦ佸厜鏁ｅ皠(綆縐幫細DLS)鏄〃寰佽剛璐ㄤ綋綺掑害鐨勪竴欏硅緝涓烘垚鐔熺殑鎶鏈傛澶栵紝鑳藉鍚屾椂閲囩敤鍔ㄦ佸厜鏁ｅ皠嫻嬮噺綺掑害銆佸茍浣跨敤嬋鍏夊鏅嫆棰戞祦閫熸祴瀹歾eta鐢典綅鐨勫垎鏋愯澶囧湪涓氬唴涔熷緱鍒版棩鐩婂箍娉涚殑搴旂敤銆?
涓嶯TA鎶鏈竴鏍鳳紝鐢辯矑瀛愬竷鏈楄繍鍔ㄤ駭鐢熺殑鍏夋暎灝勪篃鏄疍LS鎶鏈殑鏍稿績鎵鍦ㄣ侱LS鎶鏈祴閲忔暎灝勫厜寮哄害闅忔椂闂村彉鍖栦駭鐢熺殑娉㈠姩錛屽茍紜綺掑瓙鐨勬墿鏁ｇ郴鏁般傚湪姝ゅ熀紜涓婂埄鐢ㄦ柉鎵樺厠鏂?鐖卞洜鏂潶鏂圭▼灝嗘暟鎹漿鍖栦負綺掑害澶у皬鍒嗗竷鎯呭喌銆?
閲囩敤嬋鍏夊鏅嫆棰戞祦閫熸祴瀹氭硶嫻嬮噺zeta鐢典綅鏃訛紝鍚戝垎瀛愭憾娑叉垨寰矑鍒嗘暎娑叉柦鍔犵數鍦猴紝榪欎簺棰楃矑渚夸細浠ヤ竴瀹氱殑閫熺巼縐誨姩錛岃岃閫熺巼涓巣eta鐢典綅鐩稿叧銆傞氳繃嫻嬪畾璇ラ熺巼鑳藉璁＄畻鍑虹數娉寵縼縐葷巼錛屽茍鎹綆楀嚭棰楃矑鐨剒eta鐢典綅鍜寊eta鐢典綅鍒嗗竷銆?
浣跨敤椹皵鏂囦華鍣ㄥ叕鍙哥殑Zetasizer Nano緋葷粺榪涜瀹為獙錛岀粨鏋滆〃鏄庯細浣跨敤DLS綺掑害嫻嬮噺鎶鏈強嬋鍏夊鏅嫆棰戞祦閫熸祴閲弞eta鐢典綅鍚岀瓑鍙潬錛屽潎鍙傜敤浜庨槾紱誨瓙鑴傝川浣撳拰闃崇瀛愯剛璐ㄤ綋[2]銆?
鎬葷粨
綺掑害鍙妟eta鐢典綅鍙負鐗瑰畾鍖昏嵂棰嗗煙鑴傝川浣撳畾鎬у強鐮斿彂鎻愪緵閲嶈鐨勫弬鏁幫紝鍥犺岄渶瑕佺浉鍏崇殑琛ㄥ緛鎶鏈潵鎻愪緵璇︾粏鐨勮緟鍔╀俊鎭紝甯姪浜嗚В鑴傝川浣撶殑鎬ф侊紝浠庤屼紭鍖栬嵂鍓傚畾鍒跺伐鑹哄茍瀹炵幇璐ㄩ噺鎺у埗銆傛湰鏂囦粙緇嶇殑鎶鏈兘澶熸彁渚涜剛璐ㄤ綋鍒跺墏鐨勭矑搴︺佹祿搴︺亃eta鐢典綅絳夎緟鍔╀俊鎭傝嵂鐗╅厤鏂圭爺絀跺憳閫氳繃瀵硅繖浜涙ц兘鐨勪笉鏂璇侊紝灝嗕績榪涢拡瀵瑰叿浣撶殑娌葷枟鏂規閫夌敤鏈涓哄悎閫傜殑鑴傝川浣撱?
鍙傝冩枃鐚?
1. Laouini, A., Jaafar-Maalej, C., Limayem Blouza, S., Charcrosset, C & Fessi, H. Preparation, Characterization and Applications
of Liposomes: State of the Art. Journal of Colloid Science and Biotechnology Vol 1,
147-168.(2012)
2. Size and zeta potential characterization of liposomes using the Zetasizer Nano.
Malvern Instruments Application note http://www.malvern.com/en/support/resource-center/application notes/AN101104ZetaPotentialCharacterizationLiposomes.aspx (Accessed 5 May 2014)
浣滆呯畝浠?
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