棣栦釜鐪熷僵瓚呴珮鍒嗚鯨鐜囨樉寰暅 鎵撳紑鍏夎氨淇℃伅鏂板ぇ闂?/span>
2015-8-18 鏉ユ簮:涓浗鑱氬悎鐗╃綉 鐐瑰嚮嬈?/span>
鍏抽敭璇嶏細(xì)鐪熷僵 瓚呴珮鍒嗚鯨鐜?/a> 鏄懼井闀?/a>
緹庡浗鍔充雞鏂集鍏嬪姏鍥藉瀹為獙瀹ょ殑縐戝瀹朵滑寮鍙戜簡棣栦釜鐪熷僵(true-color)瓚呴珮鍒嗚鯨鐜囨樉寰垚鍍忔妧鏈紝涓虹爺絀剁粏鑳?yōu)缁撴瀯鍜岀浉鍏崇杽呮彁渚涗簡涓涓己澶х殑宸ュ叿銆傝鎶鏈皢鍏夎氨涓庤秴楂樺垎杈ㄧ巼鏄懼井鎶鏈粨鍚堣搗鏉ワ紝鍦ㄥ崟鍒嗗瓙鎴愬儚鏃跺彲浠ヨ揪鍒扮┖鍓嶇殑鍏夎氨鍜岀┖闂村垎杈ㄧ巼銆傝繖涓紿佺牬鎬ф垚鏋滃彂琛ㄥ湪鍏湀鍗佷竷鏃ョ殑Nature Methods鏉傚織涓娿?/span>
鈥滄垜浠敤榪欎竴鎶鏈嫻嬫瘡涓垎瀛愬湪絀洪棿鍜屽厜璋變腑鐨勫畾浣嶏紝鏍規(guī)嵁鍏跺厜璋卞垽鏂垎瀛愮殑棰滆壊錛屽彲浠ヨ榪欐槸棣栦釜鐪熷僵瓚呴珮鍒嗚鯨鐜囨樉寰暅錛屸濆姪鐞嗘暀鎺圞e Xu璇達(dá)紝浠栧皢榪欎竴鎶鏈懡鍚嶄負(fù)SR-STORM(spectrally resolved stochastic optical reconstruction microscopy)銆?/span>
SR-STORM鑳藉緇欏嚭姣忎釜鍒嗗瓙鐨勫厜璋卞拰絀洪棿淇℃伅錛屼負(fù)浜轟滑鎵撳紑浜嗕竴鎵囨柊鐨勫ぇ闂ㄣ傝鎶鏈笉浠呰兘澶熷湪緇嗚優(yōu)涓垚鍍忓涓粍鍒嗭紝榪樿兘媯(gè)嫻嬪眬閮ㄧ殑鍖栧鐜(姣斿pH鍙樺寲)銆傛洿閲嶈鐨勬槸錛孲R-STORM鏄竴縐嶉珮閫氶噺鎶鏈紝鑳藉湪澶х害浜斿垎閽熷唴鑾峰緱澶ч噺鍗曞垎瀛愮殑絀洪棿鍜屽厜璋變俊鎭?/span>
SR-STORM鏄疿u鍗氬＋鍩轟簬鑷繁涔嬪墠鐨勫伐浣滃紑鍙戝嚭鏉ョ殑錛屽綋鏃朵粬鍦ㄨ憲鍚嶅鑰呭簞灝忓▉(Xiaowei Zhuang)瀹為獙瀹や粠浜嬪崥澹悗鐮旂┒銆傚簞灝忓▉鏁欐巿鐮斿彂鐨勮秴楂樺垎杈ㄧ巼鎴愬儚鎶鏈疭TORM涓庤濂栧緱涓籈ric Betzig鐨勬垚鏋滀笉鐩鎬集浠詫紝鍗村拰2014騫寸殑璇鴻礉灝?dāng)鍖栧鎿﹁偐鑰岃繃銆?/span>
鐜版湁鐨勮秴楂樺垎杈ㄧ巼鏄懼井鎶鏈笉鑳界粰鍑哄厜璋變俊鎭紝榪欐牱鐨勪俊鎭浜庣悊瑙ｅ垎瀛愯涓烘槸寰堟湁甯姪鐨勶紝鑰屼笖鑳藉瀵瑰涓澏鏍囧疄鐜伴珮璐ㄩ噺鐨勫鑹叉垚鍍忋俋u鍗氬＋鍜屽悓浜嬩滑緇忚繃娣卞叆鎺㈢儲(chǔ)錛岀粓浜庤В鍐充簡榪欎竴闅鵑銆備粬浠敤鍙戝皠娉㈤暱鐩歌繎鐨?4縐嶆煋鏂欏鏍鋒湰榪涜鏌撹壊銆傚敖綆¤繖浜涙煋鏂欑殑鍏夎氨褰兼閲嶅彔錛屼絾SR-STORM鑳藉寰堝ソ鐨勫皢鍏跺尯鍒嗗紑銆傜爺絀朵漢鍛樿繕鐢ㄥ洓縐嶆煋鏂欏綰跨矑浣撱佸井綆＄瓑鍥涗釜涓嶅悓鐨勪簹緇嗚優(yōu)緇撴瀯榪涜鏍囪銆傜爺絀舵樉紺猴紝SR-STORM鑳藉鏍規(guī)嵁鍒嗗瓙鐨勫厜璋辮交鏉懼垎杈ㄤ笉鍚岀殑棰滆壊錛屾瘡涓簹緇嗚優(yōu)緇撴瀯閮借兘椴滄槑鐨勫憟鐜板嚭鏉ャ?/span>
鈥滄垜浠互澶х害10nm鐨勯珮鍒嗚鯨鐜囷紝鎴愬儚浜嗙粏鑳?yōu)鍐呭洓涓敓鐗╁缁勫垎鐨劷I洪棿浜掍綔錛屸漍u璇淬傗滅洰鍓嶈繖涓鎶鏈富瑕佺敤浜庡熀紜鐮旂┒鍜岀粏鑳?yōu)鐢熺墿瀛﹂鍩熷Q屾垜浠笇鏈涙棩鍚庝篃鑳藉皢鍏剁敤浜庡尰鐤椼傜爺絀惰呬滑鍙互鍦⊿R-STORM鐨勫府鍔╀笅瑙傚療緇嗚優(yōu)緇撴瀯鐨勫緩绔嬶紝浠ュ強(qiáng)瀹冧滑鍦ㄧ柧鐥呬腑鍙戠敓鐨勫彉鍖栥傗?/span>
鈥滅粏鑳?yōu)楠ㄦ灦鍖呮嫭涓緋誨垪鐩鎬簰浣滅敤鐨勪簹緇嗚優(yōu)緇撴瀯鍜岃泲鐧斤紝榪欎竴鎶鏈彲浠ラ氳繃絀哄墠鐨勯鑹查氶亾鍜岀┖闂村垎杈ㄧ巼錛屾彮紺轟笉鍚岄澏鏍囦箣闂寸殑浜掍綔銆傗?/span>
Xu鍗氬＋姝ｅ湪灝濊瘯榪涗竴姝ユ敼鑹繖涓鎶鏈紝浣垮畠鑳藉鐢ㄤ簬甯歌鏄懼井緋葷粺銆備粬涔熷湪寮鍙戝悎閫傜殑鏌撴枡鍜屾帰閽堬紝鍦ㄧ撼綾沖昂搴︿笂鐩戞帶緇嗚優(yōu)鍐呭眬閮ㄧ幆澧冪殑鍙樺寲錛屾瘮濡俻H鍊箋?/span>
娉細(xì)鏈綉杞澆鍐呭鍧囨敞鏄庡嚭澶勶紝杞澆鏄嚭浜庝紶閫掓洿澶氫俊鎭箣鐩殑錛屽茍涓嶆剰鍛崇潃璧炲悓鍏惰鐐規(guī)垨璇佸疄鍏跺唴瀹圭殑鐪熷疄鎬с?/div>
錛堣矗浠葷紪杈戯細(xì)xu錛?
