Characterization of DNA-loaded porous polyethersulfone particles prepared by phase inversion technique
In this study, DNA-loaded polyethersulfone (PES) porous particles were prepared by means of a liquid-liquid phase separation technique. The porous structure parameters and the diameter of the particles were then determined. It was found that the PES concentration strongly affects the porous structure and the specific surface area of the particles. By manipulating the preparation conditions, it is possible to control the structure of the particles. The DNA-loaded PES microspheres prepared using the phase inversion method are stable in water and an aqueous NaCl solution, while the DNA is hydrolyzed under acidic conditions. The DNA-loaded PES microspheres effectively accumulate harmful DNA-intercalating pollutants and endocrine disruptors, such as ethidium bromide and acridine orange, and have the potential to be used in environmental