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A versatile platform

allowing capture and detection of

normal and dysfunctional cells on the same patterned surface is

important for accessing of cellular mechanism, developing diagnostic assays and

implementing the therapy. Here, we

reported an original and

effective method for fabricating binary polymer pattern for controlled cell adhesion. The

binary polymer pattern, composed of poly (N-isopropylacrylamide)

(PNIPAAm) and poly [poly (ethylene glycol) methyl

ether methacrylate] (POEGMA) chains, was simply obtained via a

combination of surface-initiated photo-polymerization (SIPP) and surface

activated free radical polymerization (SAFRP). This method is

unique in that it does not utilize any protecting groups or procedures of

backfilling with immobilized initiator. We

demonstrated that the precise and

well-defined binary polymer patterns with high resolution were fabricated using this

facile method.