Novel water-soluble, antibacterial, and biocompatible quaternized chitosan by introducing sulfobetaine
作者：Yuxiang Chen, Jianna Li, Qingqing Li, Yuanyuan Shen, Zaochuan Ge, Wenwen Zhang, Shiguo Chen*.
關鍵字：Chitosan,Biomaterials,Biocompatibility,Antibacterial,Zwitterion
論文來源：期刊
具體來源：Carbohydrate Polymers
發表時間：2016年
Chitosan (CS) has attracted much attention
due to its good antibacterial activity and biocompatibility.However, CS is
insoluble in neutral and alkaline aqueous solution, limiting its biomedical
application tosome extent. To circumvent this drawback, we have synthesized a
novel N-quaternary ammonium-O-sulfobetaine-chitosan (Q3BCS) by introducing
quaternary ammonium compound (QAC) and sulfobetaine,and its water-solubility,
antibacterial activity and biocompatibility were evaluated compare to
N-quaternary ammonium chitosan and native CS. The results showed that by
introducing QAC, antibacterialactivities and water-solubilities increase with
degrees of substitution. The largest diameter zone of inhi-bition (DIZ) was
improved from 0 (CS) to 15 mm (N-Q3CS). And the water solution became
completelytransparent from pH 6.5 to pH 11; the maximal waters-solubility was
improved from almost 0% (CS) to 113% at pH 7 (N-Q3CS). More importantly, by
further introducing sulfobetaine, cell survival rate ofQ3BCS increased from 30%
(N-Q3CS) to 85 % at 2000 μg/ml, which is even greater than that of
nativeCS. Furthermore, hemolysis of Q3BCS was dropped sharply from 4.07%
(N-Q3CS) to 0.06%, while thewater-solution and antibacterial activity were
further improved significantly. This work proposes anefficient strategy to
prepare CS derivatives with enhanced antibacterial activity, biocompatibility
andwater-solubility. Additionally, these properties can be finely tailored by
changing the feed ratio of CS,glycidyl trimethylammonium chloride and
NCO-sulfobetaine