[Langmuir] Hierarchical Flower-like Gold Nanoparticles Labeled Immunochromatography Test Strip for Highly Sensitive Detection of Escherichia coli O157:H7
作者：Lei Zhang, Jinyun Wang, Yun Rong, Weihua Lai, Youju Huang*, Tao Chen*
關鍵字：Gold nanoparticles; Lateral-flow test strip immunoassay; Hierarchical nanostructures; Escherichia coli O157:H7
論文來源：期刊
具體來源：Langmuir, 2015, 31, 5537-5544
發表時間：2015年
Gold nanoparticles (AuNPs) labeled lateral-flow test strip immunoassay (LFTS) has been widely used in biomedical, feed/food and environmental analysis
fields. Conventional ILFS assay usually uses spherical AuNPs as labeled probes
and shows low detection sensitivity, which further limits its widespread practical
application. Unlike spherical AuNP used as labeled
probe in conventional ILFS, in our present study, a hierarchical flower-like
AuNP specific probe was designed for
LFTS and further used to detect Escherichia coli O157:H7 (E. coli O157:H7). Three types of hierarchical flower-like AuNPs, such as tipped flower-like,
popcorn-like and large-sized flower-like AuNPs were synthesized in a one-step
method. Compared with other two kinds of Au particles, tipped flower-like AuNPs
probes for LFTS particularly exhibited highly sensitive detection of E. coli O157:H7. The
remarkable improvement of detection sensitivity of tipped flower-like AuNPs probes can be achieved even as low as 103 colony-forming
units (CFU)/mL by taking advantages of its appropriate size and
hierarchical structures, which is superior over the detection performance of conventional
LFTS. Using this novel tipped flower AuNPs probes, quantitative detection of E. coli O157:H7 can be obtained partially in a wide concentration range with good repeatability. This hierarchical
tipped flower shaped AuNPs probe for LFTS is promising for the practical applications
in widespread analysis fields.