[Scientific Report] Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol
作者：Jingyun Wang, Lei Zhang, Youju Huang,* A Dandapat, GG Zhang, JW Zhang,Weihua Lai* and Tao Chen*
關鍵字：Au-Ag NPs, Polymer modification, Immunochromatography Strip
論文來源：期刊
具體來源：Scientific Report, 2017, 7, 41419
發表時間：2017年
The probe materials play a significant
role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic
test strip (ICTS). Unlike conventional ICTS assay usually uses single-component,
solid noble metal particles (e.g., gold nanoparticles) as labeled probes, in
our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS
was successfully developed for the detection of clenbuterol (CLE).
The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were
synthesized by varying the volume ratio of [HAuCl4]: [Ag NPs] via the galvanic
replacement reaction. The surface of hollow Ag-Au NPs was functionalized with
11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE
monoclonal antibody to acquire highly stable hollow Au-Ag NPs probe.
Overall size of the Au-Ag NPs, size of the holes within individual NPs and also
Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection
signals and the quantitative data (optical densities) recorded using a bioassay
reader. The sensitivity of optimized hollow Au-Ag NPs
probes for the detection of CLE has been
achieved even as low as 2 ppb in a short time (within 15 min), which is
superior over the detection performance of conventional test strip using Au NPs. The optimized hollow
Au-Ag NPs labeled test strip can be used as an ideal candidate
for the rapid screening of CLE in food samples.