JACS--Semisynthetic tellurosubtilisin with glutathione peroxidase activity
writer：Mao, SZ; Dong, ZY; Liu, JQ; et al.
keywords：Tellurosubtilisin, Glutathione peroxidase
source：期刊
Issue time：2005年
Reaction of the hydroxyl group of serine-221 of subtilisin with phenylmethanesulfonylfluoride followed by nucleophilic substitution with sodium hydrogen telluride, a semisynthetic telluroprotein, tellurosubtilisin, was prepared. Tellurosubtilisin, which displays high substrate specificity for aromatic thiols, exhibits remarkable peroxidase activity and catalyzes the reduction of hydrogen peroxide by 3-carboxy-4-nitrobenzenethiol 20?000 times more efficiently than diphenyl diselenide.