A mussel-inspired double-crosslinked tissue adhesive intended for internal medical use
作者：Changjiang Fan, Jiayin Fu, Wenzhen Zhu, Dong-An Wang
關鍵字：Tissue adhesive, Dopamine
論文來源：期刊
發表時間：2016年
It has been a great challenge to develop aldehyde-free tissue adhesives that can function rapidly and controllably
on wet internal tissues with fine adhesion strength, sound biocompatibility and degradability.
To this end, we have devised a mussel-inspired easy-to-use double-crosslink tissue adhesive (DCTA)
comprising a dopamine-conjugated gelatin macromer, a rapid crosslinker (namely, Fe3+), and a longterm
acting crosslinker (namely, genipin). As a mussel-inspired gluing macromer, dopamine is grafted
onto gelatin backbone via an one-step reaction, the catechol groups of which are capable of performing
strong wet adhesion on tissue surfaces. By addition of genipin and Fe3+, the formation of catechol–Fe3+
complexation and accompanying spontaneous curing of genipin-primed covalent crosslinking of gluing
macromers in one pot endows DCTA with the double-crosslink adhesion mechanism. Namely, the reversible
catechol–Fe3+ crosslinking executes an controllable and instant adhesive curing; while genipininduced
stable covalent crosslinking promises it with long-term effectiveness. This novel DCTA exhibits
significantly higher wet tissue adhesion capability than the commercially available fibrin glue when
applied on wet porcine skin and cartilage. In addition, this DCTA also demonstrates fine elasticity, sound
biodegradability, and biocompatibility when contacting in vitro cultured cells and blood. In vivo biocompatibility
and biodegradability are checked and confirmed via trials of subcutaneous implantation in
nude mice model. This newly developed DCTA may be a highly promising product as a biological glue
for internal medical use including internal tissue adhesion, sealing, and hemostasis.