Natural polysaccharides promote chondrocyte adhesion and proliferation on magnetic nanoparticle/PVA composite hydrogels
作者：Ruixia Hou, Lei Nie, Gaolai Du, Xiaopeng Xiong, and Jun Fu*
關(guān)鍵字：polysaccharides, magnetic, hydrogels
論文來源：期刊
具體來源：Colloids and Surface B: Biointerfaces
發(fā)表時(shí)間：2015年
This paper aims to investigate the synergistic effects of
natural polysaccharides and inorganic nanoparticles on cell adhesion and growth
on intrinsically cell non-adhesive polyvinyl alcohol (PVA) hydrogels. Previously, we have demonstrated
that Fe2O3 and hydroxyapatite (nHAP) nanoparticles are
effective in increasing osteoblast growth on PVA hydrogels. Herein, we blended
hyaluronic acid (HA) and chondroitin sulfate (CS), two important
components of cartilage extracellular matrix (ECM), with Fe2O3/nHAP/PVA
hydrogels. The presence of these natural polyelectrolytes dramatically
increased the pore size, and the equilibrium swelling ratio (ESR) while
remaining excellent compressive strength of hydrogels. Chondrocytes were seeded
and cultured on composite PVA hydrogels containing Fe2O3,
nHAP and Fe2O3/nHAP hybrids and Fe2O3/nHAP
with HA or CS. Confocal laser scanning microscopy (CLSM) and cell counting
kit-8 (CCK-8) assay consistently confirmed that the addition of HA or CS
promotes chondrocyte adhesion and growth on PVA and composite hydrogels.
Particularly, the combination of HA and CS exhibited further promotion to cell
adhesion and proliferation compared to any single polysaccharide. The results
demonstrated that the magnetic composite nanoparticles and polysaccharides
provided synergistic promotion to cell adhesion and growth. Such
polysaccharide-augmented composite hydrogels may have potentials in biomedical applications.
http://www.sciencedirect.com/science/article/pii/S0927776515003057