Tough biodegradable chitosan–gelatin hydrogels via in situ precipitation for potential cartilage tissue engineering
作者：Zhi-Sen Shen*, Xiang Cui, Rui-Xia Hou, Qun Li, Hong-Xia Deng and Jun Fu,*
關鍵字：chitosan, gelatin, hydrogel, cartilage
論文來源：期刊
具體來源：RSC Advances
發表時間：2015年
Tough porous chitosan-gelatin (CG)
hydrogels have been prepared through in
situ precipitation with the porosity and degradation rates tunable by
adjusting the preparation formulations. Compression tests showed a Young’s modulus of 3.25 MPa and a compressive strength of 2.15 MPa for the optimized C4G4 hydrogels, which are similar to or higher than those for human cartilages. Cyclic compression tests showed overlapping hysteresis loops for
several cycles, with the compressive toughness of about 75.8 J m-2. In vitro enzymatic
degradation results showed a degradation of 65.9% in 70 days, comparable to the regeneration
rate of engineered cartilage reported in literature.
Moreover, in vitro
cell culture expeirments showed excellent adhesion and
proliferation of human thyroid cartilage cells on these hydrogels, where the chondrocytes
function to secrete ECM. These tough and biodegradable
hydrogels may have potential applications for cartilage tissue engineering.