[Macromol. Biosci.] One-Pot Synthesis of Glycopolymer-Porphyrin Conjugate as Photosensitizer for Targeted Cancer Imaging and Photodynamic Therapy
writer：Jiawei Lu, Weidong Zhang, Lin Yuan, Wenjuan Ma, Xiao Li, Wei Lu, Yun Zhao*, Gaojian Chen*
keywords：http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300451/abstract;jsessionid=4611A2766935D16B14BF2E828260CA1F.f03t03
source：期刊
specific source：Macromol. Biosci., 2014, 14: 340–346.
Issue time：2013年
One-pot system
combining multi-reactions is used to synthesize novel
porphyrin-glycopolymer conjugates. Sodium mercury amalgam is used to
catalyze the reactions: 1) reduction of RAFT polymerized
poly(2-(methacrylamido) glucopyranose) (PMAG), 2) converting
protoporphyrin to protoporphyrinogen, 3) thiol-ene coupling reaction of
PMAG and protoporphyrinogen. The product is oxidized in the same pot to
generate the final porphyrin-PMAG conjugates. The resulting conjugates
are characterized by NMR, GPC, UV-Vis, and fluorescence spectroscopy.
Glycoparticles (≈200 nm) bearing glucose units are formed by dissolving
the conjugates in water. Glycoparticles show enhanced binding ability
toward Con A, bind K562 cells efficiently and kill these cells under
light irradiation in dose and light treatment length dependent manners,
illustrating the potential biological applications of the conjugates as
photosensitizer for cancer imaging and photodynamic therapy.
