[Macromol. Rapid Com.]Ratiometric Fluorescent pH Probes Based on Glycopolymers
writer：Zhiyun Li, Pengshan Zhang, Wei Lu, Lun Peng, Yun Zhao, Gaojian Chen
keywords：http://onlinelibrary.wiley.com/doi/10.1002/marc.201600242/abstract;jsessionid=29A4CCB03F3D78905533CACD90D07B92.f02t02?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+17th+December+2016+at+09%3A00+GMT%2F+04%3A00+EST%2F+17%3A00+SGT+f
source：期刊
specific source：: Macromolecular Rapid Communications Volume: 37 Issue: 18 Pages: 1513-1519 Published: SEP 2016
Issue time：2016年
Effectively detecting pH changes plays a critical role in exploring cellular functions and determining physiological and pathological processes. A novel ratiometric pH probe based on a glycopolymer, armored with properties of serum-stability, tumor-targeting, and pH monitoring, is designed. Random copolymers of 2-(methacrylamido) glucopyranose and fluorescein O-methacrylate are first synthesized by reversible addition fragmentation chain transfer polymerization. Acryloxyethyl thiocarbamoyl rhodamine B is then attached to the polymer chain to prepare ratiometric fluorescent pH probes via a thiol-ene reaction. The synthesized polymeric probes are characterized by NMR, gel permeation chromatography, UV–vis spectroscopy, and transmission electron microscopy, and the fluorescence responses are examined in phosphate buffer at different pHs. The cytotoxicity and confocal imaging experiments of the probes are detected using HeLa cells, demonstrating a low toxicity and superior biocompatibility for detecting pH changes in bioapplications.