Wang, S., Yuan, F., Chen, K., Chen, G., Tu, K., Wang, H., & Wang, L.-Q. (2015). Synthesis of Hemoglobin Conjugated Polymeric Micelle: A ZnPc Carrier with Oxygen Self-Compensating Ability for Photodynamic Therapy. Biomacromolecules, 16(9), 2693–2700. http://doi.org/10.1021/acs.biomac.5b00571
Photodynamic therapy (PDT) is a promising singlet oxygen (O-1(2)) mediated clinical treatment for many tumors. As the source of O-1(2), oxygen plays an important role in the curative effect of PDT. However, the facts of photochemical depletion of oxygen and the intrinsic hypoxic microenvironment of tumors remain the major challenges. In this work, a novel photosensitizer carrier with oxygen self-compensating ability was designed for PDT. It was synthesized via chemical conjugation of hemoglobin (Hb) to polymeric micelles formed by triblock copolymers of poly(ethylene glycol)-block-poly(acrylic acid)-block-polystyrene (PEG-b-PAA-b-PS). The PEG-b-PAA-b-PS and resultant micelles in aqueous solution were comprehensively characterized by means of FTIR, NMR, GPC, DLS, TEM, and fluorescence spectroscopy. The oxygen-binding capacity and antioxidative activity of the Hb conjugated micelles were evaluated via UV-vis spectroscopy. In addition, compared with the control micelles without Hb, the Hb conjugated photosensitizer carrier was able to generate more O-1(2) and exert greater photocytotoxicity on Hela cells in vitro.