In-situ sampling and monitoring ctDNA from dermal interstitial fluid for tumor diagnosis
作者：Zhenyu Wu, Jing Chen, Yuzhe Zhang, Rufei Ge, Yan Li, Khaydar E. Yunusov, Fozil Turaku
關鍵字：Electrochemical biosensor, microneedles, ctDNA, detection
論文來源：期刊
具體來源：Biosensors and Bioelectronics
發表時間：2026年
Abstract: Detecting circulating tumor DNA (ctDNA) in skin interstitial fluid (ISF) offers a non-invasive cancer diagnostic route, though clinical translation is often hindered by low extraction efficiency and sensitivity. This study presents an integrated microneedle-electrode platform for in situ sampling and monitoring of ctDNA. Utilizing reverse iontophoresis, ctDNA is extracted from the dermal ISF and subsequently detected via a signal-amplified rGO/PPy/Au NPs electrochemical sensor. The platform facilitates quantitative detection with a broad linear range (10 fM to 1 nM) and a low detection limit of 1.92 fM. It demonstrates high specificity against base mismatches and biological interferents, alongside strong reproducibility. In vivo testing on a murine tumor model showed results significantly correlated with qPCR and ddPCR, highlighting its potential for point-of-care cancer diagnostics and disease monitoring.
Keywords: Electrochemical biosensor, microneedles, ctDNA, detection
