92. Preparation of glucose-responsive and fluorescent micelles via combination of RAFT polymerization and chemenzymatic transesterification for controlled release of insulin
作者：Lei Li, Guohua Jiang,* Xiangxiang Du, Hua Chen, Yongkun Liu, Qin Huang, Xiangdong Kong and Juming Yao
關(guān)鍵字：glucose-responsive, fluorescent, micelles, insulin
論文來源：期刊
具體來源：RSC Advances, 2015, 5(92), 75766-75772.
發(fā)表時間：2015年
The glucose-responsive and fluorescent copolymer was prepared via one-pot method that combined RAFT polymerization and enzymatic transesterification. The as-prepared copolymer tended to self-assemble into spherical micelles that confirmed by 1H NMR, FT-IR and GPC, transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. Due to attachment of fluorescent side groups, the optical properties of micelles had been analysized by UV-Vis spectrometer, fluorescence spectrophotometer and confocal laser scanning microscope (CLSM). Owing to the amphiphilic and glucose-responsive properties, the micelles could be used as carriers to load insulin. And the insulin release behavious were evaluated triggered by glucose in vitro. In addition, the resultant micelles exhibited relative lower cytotoxicity and excellent stability against protein solution. The insulin remained its structure stability after release through circular dichroism (CD) spectra analysis. These results showed that the obtained glucose-responsive and fluorescent polymer micelles with good biological imaging performance and biocompatibility could be one of effective candidate carriers for controllable release of insulin.