Immobilization of Bovine Serum Albumin onto Spherical Polyelectrolyte Brushes in Aqueous Solution
作者：Wang, Siyi; Zhu, Yuanqing; Chen, Kaimin; Li, Li; Guo, Xuhong
關鍵字：無
論文來源：會議
具體來源：The 4th International Conference on BioMedical Engineering and Informatics 2011 Shanghai
發表時間：2011年
The adsorption of bovine serum albumin (BSA) with spherical poly(acrylic acid) brushes (SPB) was observed by the methods of turbidimetric titration, rheology, dynamic light scattering (DLS), Zeta potential measurement, and UV spectroscopy. Turbidimetric titration and DLS were used to examine the binding boundaries of protein-polyelectrolyte. The critical pH values where adsorption was initiated (pHc) and aggregation occurred (pHφ) were determined by turbidimetric titration and DLS, respectively. Adsorption of BSA by SPB occurred at range of 4.6 < pH < 7.0. High ionic strength can damage the binding between BSA and SPB. Upon decreasing pH value, the viscosity, the elastic modulus G’ and viscous modulus G” of SPB binding of BSA increased significantly. The binding amount of BSA in SPB was determined by UV spectroscopy.