Hierarchical-multiplex DNA Patterns Mediated By Polymer Brush Nanocone Arrays That Possess Potential Application for Specific DNA Sensing.
writer：2) Wendong Liu, Xueyao Liu, Peng Ge, Liping Fang, Siyuan Xiang, Xiaohuan Zhao, Huaizhong Shen, and B
keywords：polymer brush, DNA patterns, nanocone arrays, sensing, hierarchical-multiplex
source：期刊
specific source：ACS Appl. Mater. Interfaces 2015, 7, 24760?24771
Issue time：2015年
This paper provides a facile and cost-e?cient
method to prepare single-strand DNA (ssDNA) nanocone arrays
and hierarchical DNA patterns that were mediated by poly(2-
hydroxyethyl methacrylate) (PHEMA) brush. The PHEMA brush
nanocone arrays with di?erent morphology and period were
fabricated via colloidal lithography. The hierarchical structure was
prepared through the combination of colloidal lithography and
traditional photolithography. The DNA patterns were easily
achieved via grafting the amino group modi?ed ssDNA onto the
side chain of polymer brush, and the anchored DNA maintained
their reactivity. The as-prepared ssDNA nanocone arrays can be applied for target DNA sensing with the detection limit reaching
1.65 nM. Besides, with the help of introducing micro?uidic ideology, the hierarchical-multiplex DNA patterns on the same
substrate could be easily achieved with each kind of pattern possessing one kind of ssDNA, which are promising surfaces for the
preparation of rapid, visible, and multiplex DNA sensors.