Graded Protein/PEG Nanopattern Arrays: Well-Defined Gradient Biomaterials to Induce Basic Cellular Behaviors.
writer：Peihong Xue, Wendong Liu, Zhongyi Gu, Xingchi Chen, Jingjie Nan, Junhu Zhang, Hongchen Sun, Zhanc
keywords：gradient biomaterials, colloidal lithography, well-de?ned ECM, inducing cellular behavior, multifunctional substrate
source：期刊
specific source：ACS Appl. Mater. Interfaces
Issue time：2019年
Gradient biomaterials have shown enormous
potential in high-throughput screening of biomaterials and
material-induced cell migration. To make the screening
process more rapid and precise, improving the regularity of
morphological structure and chemical modi?cation on
gradient biomaterials have attracted much attention. In this
paper, we present a novel fabrication strategy to introduce
ordered nanopattern arrays into gradient biomaterials, through
combining surface-initiated atom transfer radical polymer-
ization and inclined reactive-ion etching based on colloidal
lithography. Graded protein/poly(ethylene glycol) (PEG)
nanopattern arrays on a quartz substrate were fabricated and
applied to a?ect the behaviors of cells. Owing to the
continuously changed ratio of two di?erent components, the corresponding cell adhesion density along the substrate showed
obvious graded distribution after culturing for 24 h. Meanwhile, the cytoskeleton showed obvious polarization after culturing for
7 days, which is parallel with the direction of gradient. Additionally, oriented migration was generated when mouse MC3T3-E1
cells were cultured on the graded protein/PEG nanopattern arrays. On the basis of the ordered and well-de?ned nanopatterns,
the correlation between the extracellular matrix and corresponding expressions generated by di?erent stimuli can be
investigated.