Enhanced Biocompatibility of PLGA Nanofibers with Gelatin/Nano-Hydroxyapatite Bone Biomimetics Incorporation.
writer：Daowei Li, Haizhu Sun, Liming Jiang, Kai Zhang, Wendong Liu, Yang Zhu, Jiaozi Fangteng, Ce Shi,
keywords：electrospun nano?bers, poly(lactic-co-glycolic acid), gelatin/nanohydroxyapatite, bone biomimetics, bone tissue engineering, biocompatibility
source：期刊
specific source：ACS Appl. Mater. Interfaces
Issue time：2014年
The biocompatibility of biomaterials is essen-
tially for its application. The aim of current study was to
evaluate the biocompatibility of poly(lactic-co-glycolic acid)
(PLGA)/gelatin/nanohydroxyapatite (n-HA) (PGH) nano-
?bers systemically to provide further rationales for the
application of the composite electrospun ?bers as a favorable
platform for bone tissue engineering. The PGH composite
sca?old with diameter ranging from nano- to micrometers was
fabricated by using electrospinning technique. Subsequently,
we utilized confocal laser scanning microscopy (CLSM) and MTT assay to evaluate its cyto-compatibility in vitro. Besides, real-
time quantitative polymerase chain reaction (qPCR) analysis and alizarin red staining (ARS) were performed to assess the
osteoinductive activity. To further test in vivo, we implanted either PLGA or PGH composite sca?old in a rat subcutaneous
model. The results demonstrated that PGH sca?old could better support osteoblasts adhesion, spreading, and proliferation and
show better cyto-compatibility than pure PLGA sca?old. Besides, qPCR analysis and ARS showed that PGH composite sca?old
exhibited higher osteoinductive activity owing to higher phenotypic expression of typical osteogenic genes and calcium
deposition. The histology evaluation indicated that the incorporation of Gelatin/nanohydroxyapatite (GH) biomimetics could
signi?cantly reduce local in?ammation. Our data indicated that PGH composite electrospun nano?bers possessed excellent cyto-
compatibility, good osteogenic activity, as well as good performance of host tissue response, which could be versatile
biocompatible sca?olds for bone tissue engineering.