【ACS Applied Materials & Interfaces】Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy
作者：Jiao Ma, Shifang Luan*, Lingjie Song, Jing Jin, Shunjie Yan, Huawei Yang, Hengchong Shi, Jinghua Yin*, et al.,
關鍵字：cycloolefin polymer (COP), surface-initiated photoiniferter-mediated polymerization (SI-PIMP), hierarchical architecture, antibody immobilization, immunoassay
論文來源：期刊
具體來源：ACS Applied Materials & Interfaces, 2014, 6, 1971-1978
發表時間：2014年
Abstract:
The development of technologies for a biomedical detection platform is critical to meet the global challenges of various disease diagnoses. In this study, an inert cycloolefin polymer (COP) support was modified with two-layer polymer brushes possessing dual functions, i.e., a low fouling poly[poly(ethylene glycol) methacrylate] [p-(PEGMA)] bottom layer and a poly(acrylic acid) (PAA) upper layer for antibody loading, via a surface-initiated photoiniferter-mediated polymerization strategy for fluorescence-based immunoassay. It was demonstrated through a confocal laser scanner that, for the as-prepared COP-g-PEG-b-PAA-IgG supports, nonspecific protein adsorption was suppressed, and the resistance to nonspecific protein interference on antigen recognition was significantly improved, relative to the COP-g-PAA-IgG references. This strategy for surface modification of a polymeric platform is also applicable to the fabrication of other biosensors.