Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy
writer：Mengyi Li, Xiaoyan Zhou, Xiaolong Zeng, Changyong Wang, Jiake Xu,Dong Ma, Wei Xue
keywords：targeting, hyperbranched poly(amido amine)s, cancer therapy
source：期刊
Issue time：2016年
For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized
by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N0-cystaminebisacrylamide.
Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched
poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection
efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in
the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis
of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than
PEI-25k, suggesting a potential application in gene therapy for tumors.