Electrospun fibrous scaffolds of poly(lactic-co-glycolic acid) containing bovine glycosaminoglycan for cartilage tissue engineering and treatment of deep cartilage defects in the knee
writer：8. Peibiao Zhang, Xin Wang, Zhi Wang, Xiuli Zhuang, Xiabin Jing, Xuesi Chen?
keywords：poly(lactic-co-glycolic acid), glycosaminoglycan,electrospinning, cartilage tissue engineering
source：會議
specific source：International Symposium on Polymer Chemistry PC 2006, Dalian, June 15-19,
Issue time：2006年
Tissue engineering provides an alternative approach to restoring, maintaining or improving the function of human tissues by natural and synthetic biomaterials seeded with available cells. Synthetic polymers, mainly polyesters such as poly(L-lactic acid )(PLLA), poly(glycolic acid) (PGA) and their copolymers (PLGA), have shown potential applications in tissue engineering with unlimited availability, higher mechanical property, and biodegradability. They are easily and reproducibly processed into a desired shape and structure, which can be maintained after implantation. Glycosaminoglycans (GAGs) is a component of proteoglycans (PGs), which are most abundant in cartilage and account for the ability to sustain high repetitive compressive loads.
To observe the roles of GAGs in cell growth and differentiation, and tissue regeneration, a kind of fibrous scaffolds of poly(lactic-co-glycolic acid) (PLGA) containing 0.1-10% (w/w) bovine GAGs was fabricated with electrospinning for cartilage tissue engineering. The scaffolds were analyzed by SEM, AFM, TGA and DSC. Chondrocytes from articular cartilage of new-born rabbits were obtained and cultured in vitro. Freshly isolated chondrocytes were seeded on the disk of GAGs/PLGA fibrous scaffold. Cell viability was examined by MTT method. Tissue engineered cartilages of sandwich structure with GAGs/PLGA and allogenic chondrocytes were constructed by centrifugation and harvested after 2 to 4 weeks culture, and grafted to deep cartilage defects in rabbit knees. Biopsies in 1w to 24w were obtained and analyzed by histomorphology, in situ hybridization and immunohistochemistry.