Photo-immobilization of Bone Morphogenic Protein 2 on PLGA/HA Nanocomposite to Enhance the Osteogenesis of Adipose-Derived Stem Cells.
writer：T Gao, W Cui,Z Wang,Y Wang,Y Liu,PS Malliappan,Y Ito and P Zhang.
keywords：Photo-immobilization,Nanocomposite,Adipose-Derived Stem Cells, Tissue engineering
source：期刊
specific source：RSC Adv., 2016, 6, 20202-20210.
Issue time：2016年
Abstract
Lack of bioactivity has seriously
restricted the development of biodegradable implants in bone tissue engineering.
In this study, Bone Morphogenic Protein 2 (BMP-2) was photo-immobilized on the
poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA composites
via photo-reactive gelatin (Az-Gel). The results demonstrate that the Az-Gel
facilitates efficient and long-term immobilization of peptides on the surface
of PLGA. The immobilization of Az-Gel enhanced the adhesion and proliferation
of Mouse Adipose-Derived Stem Cells (ADSCs), while HA incorporation facilitated
cellular osteodifferentiation. The immobilization of BMP-2 induced the
osteogenesis of the ADSCs, indicated by alkaline phosphatase activity,
quantitative Real-Time polymerase chain reaction (qRT-PCR) analysis and
mineralization on the deposited substrates. Furthermore, the surface immobilization
of BMP-2 could not only reduce the amount of growth factor, but also perform
long-term osteoinductive effect. Therefore, it is considered that the surface
modification method possessed a great potential for enhancement of
osteointegration of biodegradable bone implants.
Scheme 1.Schematic illustration of Az-Gel-assisted
immobilization of BMP-2 on PLGA/HA scaffolds for enhancing proliferation and
osteogenic differentiation of ADSCs
全文：
http://pubs.rsc.org/en/content/articlelanding/2016/ra/c5ra27914c#!divAbstract