Biodegradable and intelligent conducting polymer for tissue engineering application
作者：4. Peibiao Zhang1, Wei Zhang1,2, Lihong Huang1, Xiuli Zhuang1, Xuesi Chen1*, and Xiabin Jing
關鍵字：Conducting polymer, tissue engineering
論文來源：會議
具體來源：International Symposium on Polymer Chemistry, PC2008, Hefei／2008. 06. 15-19
發表時間：2008年
Tissue and organ failure, caused from injury or other type of damage, is a major health problem. The traditional treatments include organ transplantation, surgical reconstruction and mechanical or synthetic devices. Tissue engineering is emerging as a promising alternative or complementary solution for therapy of tissue and organ failure. The synthetic biomaterials, such as poly(lactic acid )(PLA), poly(glycolic acid)(PGA) and their copolymers(PLGA), play an important role in construction of engineered tissue. The problems related to tissue engineering are materials lack of response to environment and the interaction of electrophysiology among cells in scaffolds or devices.
Electrically conductive polymers, such as polyacetylene, polypyrrole (PPy), polythiophene, polyaniline (PANi), and poly(para-phenylene vinylene), have been proved most effective in improving a multitude of cell functions, such as cell attachment, proliferation, migration, and differentiation through electrical stimulation. However, toxicity and undegradability of conductive polymers are critical limitation in application of tissue engineering. To solve these problems, PLA-b-AP-b-PLA (PAP) triblock copolymer of PLA and aniline pentamer (AP) with improved biocompatibility, biodegradability, solubility and processability was synthesized in our group. A primary investigation was undertaken with cell culture and assistance of electrical stimulation for its application in cardiac tissue engineering. Biocompatibility of PAP copolymer was investigated by cytotoxicity test, and cell attachment and morphological analysis. The effects of electrical stimulation on myoblasts (H9c2 cell lines) cultured on the thin film surface of the PAP/PLA (1:1) were subsequently investigated by cell proliferation test and ion image analysis.
The survival rate of C6 cells in PAP copolymer extract stock solution is higher than those in AP and PLA/AP (1:1). The area fraction and perimeter of H9c2cells on PAP/PLA film surface were the highest, and circularity was the lowest at each time point which proved that the copolymer has favorable biocompatibility. After electrical stimulation (5V, 1Hz, 500ms), the proliferation ratio and intracellular calcium concentration of H9c2 cells on PAP/PLA film surface