Poly(ethylene glycol)-cross linked Poly(methyl vinyl ether-co-maleic acid) hydrogels for Three-Dimensional Human Ovarian Cancer Cell Culture
作者：Tianzhu Zhang*, Junsong Chen, Qiying Zhang, Jun Dou, and Ning Gu*
關鍵字：3D culture, Multicellular spheroid, Human ovarian cancer cell, Hydrogel, Poly(methyl vinyl ether-co-maleic acid), Rheological properties
論文來源：期刊
具體來源：Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013, 422: 81–89
發表時間：2013年
In this work, three kinds of poly(ethylene glycol)-cross linked poly(methyl vinyl ether-co-maleic acid) hydrogels, Gel-1, Gel-2, and Gel-3 with different elastic modulus of 105,100 Pa, 12,020 Pa, and 2191 Pa were designed for simulating respectively the mechanical strength of stiffer metastasis bone sites, cancerous soft tissue, and BME or collagen, and were evaluated in three-dimensional (3D) culture of human ovarian cancer cell line HO8910. The proliferation, adhesion, migration, and invasion of ovarian cells HO8910 in these three kinds of hydrogels were systematically investigated and compared with two commercial hydrogels BME and collagen I. Hydrogels Gel-1 and Gel-2 with higher elastic modulus showed a better cytocompatibility and remarkably supported the proliferation of ovarian cancer cell line HO8910 and the formation of multicellular spheroids (MCS) in a 3D model. The mechanical properties of hydrogels played a crucial role in responses of HO8910 cells. The hydrogels Gel-1 and Gel-2 are also the potential 3D culture matrix materials for cancer cell culture.