A colorimetric sensor for determination of cysteine by carboxymethyl cellulose-functionalized gold nanoparticles
作者：Wei XY, Qi L, Tan JJ, Liu RG, Wang FY
關鍵字：Gold nanoparticles; Carboxymethyl cellulose; Cysteine; Colorimetric sensor; Urine
論文來源：期刊
具體來源：Analytica Chimica Acta 2010, 671(1-2), 80-84
發表時間：2010年
A simple and sensitive colorimetric method for cysteine detection was established based on the carboxymethyl cellulose-functionalized gold nanoparticles (CMC-AuNPs). The nanoparticles were directly synthesized with sodium carboxymethyl cellulose by a simple approach, which would protect particles against salt-induced aggregation. Then the CMC-AuNPs solution exhibited a high colorimetric selectivity to cysteine. The assay results indicated that the introduction of cysteine could induce the aggregation of the colloidal solutions at the presence of sodium chloride, displaying changes in color and in UV-vis absorption spectra. Thus an exceptionally simple, rapid method for detecting cysteine was obtained at the linear range of 10.0-100.0 mu M with the relative coefficient of 0.997. The proposed method possessed the advantages of simplicity and sensitivity, and was applied to real urine sample detection. The results were satisfying and the proposed method was especially appropriate for detection of cysteine in biological samples.