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Dual-color monodisperse polystyrene microspheres have been prepared by two-stage dispersion polymerization,whose addition of the dye-
monomer was deferred until the nucleation stage was complete.Two dyes were covalently incorporated into microspheres by copolymerization
ithout disturbing the final particle size and size distribution.No dye leakage occurs for the microsphere suspension because of covalent bonding
 dye molecules.By varying the dye concentrations in microspheres,we obtained fluorescent microspheres with two distinguishing parts of
uorescence and varied fluorescent intensity ratios under a single-wavelength excitation.The microspheres present intense fluorescence for the
rge amount of encapsulated dye molecules.