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writer：Yun-Pu Wang, Na-Li Chen, Rong-Min Wang, Yu-Bing Xiong, Rui-Tao Zhay
keywords：poly(sodium acrylate); living cell Immobilization; bacterial Fertilizer
source：期刊
specific source：POLYMERS FOR ADVANCED TECHNOLOGIES
Issue time：2004年

A living azospirillum simplex cell (LASSC) was immobilized on poly(sodium acrylate) (PSA). The method of preparation of the immobilized-cell (LASSC-PSA) was studied. LASSC was immobilized on PSA in an immobilizing medium of Na₂HPO₄ ^. 12H₂O-NaH₂PO₄^.2H₂O (pH 7.0) buffer, sterile water, a solution of NaCl (0.85% m/m), cultured medium (pH 7.0), respectively. The desorption capacity of LASSC on LASSC-PSA in sterile water was measured and when the Na₂HPO₄ ^.12H₂O-NaH₂PO₄ ^.2H₂O (pH 7.0) buffer served as the immobilizing medium, the desorption capacity of LASSC on LASSC-PSA was the highest. The rate (m/m) of PSA and LASSC (wet weight) also affected considerably the desorption capacity of LASSC on LASSC-PSA in sterile water, and the best rate was 1 : 0.5. The desorption capacity of LASSC on LASSC-PSA was 50% of that at the beginning and that was still 109 cfu/g on storage for 5 months when the rate (m/m)
of PSA and LASSC (wet weight) was 1 : 0.5, using Na₂HPO₄^. 12H₂O-NaH₂PO₄ ^. 2H₂O (pH 7.0) buffer as immobilizing medium.