The preparation of nano-scope chitosan-oligomer copper complexes and their interaction with DNA
作者：Rong-Min Wang, Nai-Pu He, Yu-Feng He, Yun-Tao Xie, Yun-Pu Wang,Eishun Tsuchida
關(guān)鍵字：chitosan; nanocomposites; biological applications of polymers; DNA; copper complexes
論文來(lái)源：期刊
具體來(lái)源：POLYMERS FOR ADVANCED TECHNOLOGIES
發(fā)表時(shí)間：2005年
Water-soluble low molecular weight chitosan of nanometer level and its copper complexes were prepared, and characterized by IR spectra, elemental analysis and gel permeation chromatography (GPC). The modes and mechanism of these copper complexes interaction with DNA were studied by a fluorescent probe method and electrophoresis analysis. It is suggested that there are electrostatic and intercalation modes of copper complexes interacting with DNA. At first, the cationic complex electrostaticly binds to the negatively charged phosphate backbone of DNA, and then a portion of the complex intercalates between the base pairs on the DNA duplex strand.