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For efficiently sieving active molecules to concentrate/purify antibiotics, the pores of novel hydrophilic

polyethylene glycol (PEG) based nanofiltration (NF) membrane should be finely tailored. Herein, an effective coating layer is successfully built on the PEG based NF membrane through self-polymerization of

mussel-inspired dopamine (DA) which is confirmed by various physicochemical characterizations. Interestingly, with smaller pores, the hydrophilic PEG based membranes after coating with mussel-inspired

polydopamine can significantly increase the rejections to salts and other active molecules with only little

sacrifice of dual resistance to fouling and chlorine. Importantly, the best comprehensive antibiotics separation performance of NF membrane can be achieved by mussel-inspired dopamine modification

around 6 h. Such an advanced membrane shows the highly stable tobramycin solution flux over

46 L m

2

h

1

alongside tobramycin (TOB), clindamycin phosphate (CP) and cephalexin (CA) rejections

up to 99%, 94% and 93% with 50 ppm feed concentration under 8.0 bar, respectively. Interestingly, when

the feed concentration increases up to 800 ppm, the membrane still exhibits the high rejections to all of

antibiotics up to 90%. Therefore, coating with mussel-inspired dopamine is a highly effective way to tailor

the pores of hydrophilic PEG based NF membranes toward the excellent separation performance for

expanding membrane applications in separating negative and amphiprotic charged antibiotics