Microbial Cells with a Fe3O4 Doped Hydrogel Extracellular Matrix: Manipulation of Living Cells by Magnetic Stimulus
writer：Xudian Shi, Zhijun Shi, Daming Wang, Muhammad Wajid Ullah, Guang Yang*
keywords：Microbial Cells,Fe3O4 Doped,Extracellular Matrix
source：其他
specific source：Macromolecular Bioscience， 2016, 16, 1506-1514
Issue time：2016年
This study aims to develop an effective method to control motile microorganisms and enable
their manipulation as functional ‘live micro/nano robots’. A novel strategy based on Fe3O4
nanoparticle-doped alginate hydrogel is developed to fashion an artificial extracellular matrix
(ECM) for microbial cells (e.g., Saccharomyces cerevisiae and Flavobacterium heparinum).
During this strategy, a single layer of alginate hydrogel is coated around the microbial cells
doped with Fe3O4 nanoparticles to form the alg-mag-cells. Transmission electron microscopy
shows that Fe3O4 nanoparticles are uniformly distributed in the hydrogel shell. Together
with maintaining the cell activity and metabolism, the hydrogel coated microbial cells demonstrate
high magnetic responsiveness in an external magnetic
field and are able to form micro-scaled patterns using
the magnetic template designed in this study. This strategy
provides a building block to fabricate advanced biological
models, medical therapeutic products, and non-medical biological
systems using different microorganisms.