電活性有序微納界面誘導(dǎo)皮膚組織修復(fù)的機(jī)理研究
項(xiàng)目類(lèi)別：國(guó)家自然科學(xué)基金
項(xiàng)目編號(hào)：21574050
項(xiàng)目規(guī)模：面上項(xiàng)目
參與人員：楊光（主持）、石志軍、黃芳、李瑩、黃亞佳、劉莉、李思祥、曾迪
起止日期：2016.01-2019.12
生物體產(chǎn)生的內(nèi)源信號(hào)在發(fā)育、再生以及自我修復(fù)過(guò)程起著舉足輕重的作用。本項(xiàng)目
采用已建立的生物制造過(guò)程控制的方法，在生物合成過(guò)程中經(jīng)分子模板和微流控調(diào)控葡糖
醋桿菌的定向運(yùn)動(dòng)，此外通過(guò)構(gòu)建趨光型葡糖木醋桿菌工程菌株，控制纖維素纖維的組裝
與排列，光控合成具有特定規(guī)則圖案的三維的纖維素材料，為組織細(xì)胞提供多層次高度精
細(xì)結(jié)構(gòu)的組織工程支架，誘導(dǎo)其與支架的定向黏附和聚集。進(jìn)一步通過(guò)電活性修飾，模擬
生物體的內(nèi)源生物電信號(hào)，引導(dǎo)和調(diào)控成纖維細(xì)胞、神經(jīng)元細(xì)胞、內(nèi)皮細(xì)胞的分布、遷移
、增殖、分化等。本項(xiàng)目針對(duì)“調(diào)控內(nèi)源信號(hào)實(shí)現(xiàn)無(wú)損定向誘導(dǎo)皮膚組織的修復(fù)與再生”
的關(guān)鍵科學(xué)問(wèn)題。采用在有序微納界面上模擬內(nèi)源電信號(hào)刺激的方法，構(gòu)建基于有序微納
界面與生物電信號(hào)的定向誘導(dǎo)組織修復(fù)與再生的模型，在分子、細(xì)胞、組織等水平闡明微
納界面誘導(dǎo)和內(nèi)源生物電信號(hào)的協(xié)同作用機(jī)制，為實(shí)現(xiàn)皮膚組織的定向修復(fù)與再生提供科
學(xué)依據(jù)。
Endogenous electric signal is fundamentally important for development,
regeneration, and wound healing. This project adopts the recently established
bio-manufacturing and process control methods to control the directed movement
of Gluconacetobacter xylinum through molecular template and microfluidics. In
addition, by constructing engineered phototaxic Gluconacetobacter xylinum,
assembly and arrangement of cellulose fibers is light controlled to achieve a
specific three-dimensional micro-pattern, which will provide highly
sophisticated multi-level tissue engineering scaffolds to induce directed cell
adhesion and aggregation, for regenerative medicine purposes Further, the
micro-patterned materials will be locally modified with conductive materials
to simulate the endogenous electrical signaling to induce and modulate the
distribution, migration, proliferation, and differentiation of fibroblasts or
neurons. t. This project aims to solve the key scientific issue concerning how
to achieve directed induction of skin repair and regeneration through
modulation of endogenous signaling by simulating the endogenous electric
signal on a patterned micro-/nano-surface and also to elucidate the underlying
mechanisms of the additive effect of micro-/nano-surface and biological
electric signal at molecular, cellular, and tissue level. This will provide a
solid scientific basis for directed repair and regeneration of skin tissues.