[Soft Matter] Soft Landing of Cell-Sized Vesicles on Solid Surfaces for Robust Vehicle Capture/Release
作者：Dehui Wang, Zhengfang Wu, Aiting Gao, Weihong Zhang, Chengying Kang, Qi Tao, Peng Yang*
關鍵字：Lysozyme, Vesicles, Lipid Bilayer, Surface Modification, Electrostatic Self-Assembly
論文來源：期刊
具體來源：Soft Matter
發表時間：2015年
Soft Matter, 2015, DOI: 10.1039/C5SM00049A
Based
on a concept of a smooth and steady landing of fragile
objects without destruction via a soft cushion, we have developed a model for
the soft landing of deformable lipid giant unilamellar vesicles (GUVs) on solid
surfaces. The foundation for a successful soft landing is a solid substrate
with a two-layer coating including a bottom layer of positively charged
lysozymes and an upper lipid membrane layer. We came to the clear conclusion
that when anionic GUVs sedimented on a surface, the vesicle rupture occurred
upon direct contact with the positively charged lysozyme layer due to strong
Coulombic interactions. In contrast, certain separating distances achieved by
the insertion of a soft lipid membrane cushion between the charged GUVs and the
lysozyme layer attenuated the Coulombic force and created a mild buffer zone,
ensuring the robust capture of GUVs on the substrate without their rupture.
The non-covalent bonding facilitated a fully reversible stimuli-responsive
capture/release of GUVs from the biomimetic solid surface, which has never
before been demonstrated due to the extreme fragility of GUVs. As a matter of
fact, the controllable capture/release of cells has been proven to be of vital
importance in biotechnology, and the similarity of the present approach to cell
capture/release is expected to open previously inaccessible avenues of
research.