Determination of Molecular Weight of Silk Fibroin by Non-Gel Sieving Capillary Electrophoresis
作者：Wei Wei, Yaopeng Zhang, Huili Shao, Xuechao Hu
關(guān)鍵字：non-gel sieving capillary electrophoresis , silk fibroin, spider silk, silk worm, molecular weight
論文來源：期刊
具體來源：Journal of AOAC INTERNATIONAL, 2010 (In press).
發(fā)表時間：2010年
A simple non-gel sieving capillary electrophoresis (NGSCE) method was established to determine molecular weight (MW) of silk fibroin using capillary electrophoresis system (CE). The background electrolyte with a pH of 8.8 was based on the three components polyethylene glycol, tris(hydroxymethyl)aminomethane (Tris) and sodium dodecyl sulfate (SDS). NGSCE showed a good linear relationship with fine reproducibility between the migration time and the MW of standard proteins. It was found that the regenerated silk fibroin had a MW around 83 kDa with a wide molecular weight distribution (MWD). This absolute value is lower than the result obtained from the Lauryl sodium sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) due to their different principles, but their similar MWD shapes indicated that NGSCE could be a feasible, highly sensitive, rapid method for the determination of MW of silk fibroin.