Physicochemical characterization of high-quality bacterial cellulose produced by Komagataeibacter sp. strain W1 and identification of the associated genes in bacterial cellulose production
作者：Shan-Shan Wang; Yong-He Han; Yu-Xuan Ye; Xiao-Xia Shi; Deng-Long Chen; Min Li
關鍵字：Bacterial cellulose; Komagataeibacter sp.; Scanning electron microscopy; X-ray diffraction; Fourier transform infrared spectroscopy; Genome sequencing
論文來源：期刊
具體來源：RSC Advances
發表時間：2017年
In the last few decades, the bacteria capable of bacterial cellulose (BC) synthesis and the characterization of BC have been well-documented. In this study, a new BC-producing bacterial strain was isolated from the fermented vinegar. The BC morphology, composition and diameter distribution, and the genes associated with BC production were analyzed. The results showed that one out of five isolates belonging to Komagataeibacter was BC-producer, which mostly produced the typical cellulose I consisting of nanofibrils and had several functional groups similar to typing paper (i.e., plant cellulose). Several known genes such as glk, pgm and UPG2 in glucose metabolisms, bcsA, bcsB, bcsC and bcsD in BC synthesis and cmcax, ccpAx, bglxA and other genes in BC synthesis regulation or c-di-GMP metabolisms have also been found in the strain W1 based on genome sequencing and gene annotations. The functions of BcsX and BcxY might also be important for BC synthesis in Komagataeibacter sp. W1. Our study provided a new BC-producing bacterial strain that could be used to prepare high-quality BC and to study BC synthesis mechanisms.