49. Preparation of keratin/chlorhexidine complex nanoparticles for long-term and dual stimuli-responsive release
writer：Xuelian Zhi, Yanfang Wang, Pengfei Li, Jiang Yuan*, Jian Shen*.
keywords：Keratin
source：期刊
specific source：RSC Advance 2015, 5, 82334 - 82341
Issue time：2015年
Nanoscale polyion
complex formed by polyelectrolyte and charged drug via electrostatic
complexation is the most convenient method for building a drug delivery system to simultaneously realizes the carrier preparation
and drug embedding. Herein, we prepared keratin/chlorhexidine
complex nanoparticles (KCNPs) based on a drug-induced ionic gelation technique
without using crosslinker, organic solvent and surfactant. These KCNPs exhibited
good stability even after long-standing for 14 days. The KCNPs were
characterized with FTIR, DLS, SEM and TEM. It was found that these
nanoparticles had the spherical morphology with the diameter of about 180 nm,
and negatively charged surface with the zeta potential of about -39.1 mV. Cell
toxicity of KCNPs at different dosages level was evaluated
with MTT assay method, indicating their slight cytotoxicity at lower dosage. The
antibacterial activity against E. coli and S. aureus was determined using a zone
of inhibition method. It seemed KCNPs had better antibacterial activity against S. aureus than E. coli. Drug delivery profile showed that chlorhexidine(CHX)
loaded nanoparticles exhibited both pH and glutathione responsive. These keratin-based
complex nanoparticles can be regarded as a valuable stimuli responsive strategy
for the delivery of anticancer agents.