Injectable cell- and growth factor-free poly(4-hydroxybutyrate) (P4HB) microspheres with open porous structure and great efficiency of promoting bone regeneration
writer：Shuaishuai Yuan, Yong Shen, Zhibo Li*
keywords：poly(4-hydroxybutyrate) (P4HB), double-emulsion solvent evaporation method, open porous microsphere, bone tissue engineering, bone defect
source：期刊
specific source：ACS Applied Bio Materials
Issue time：2021年
Delivering injectable microspheres in a minimally invasive way to repair
complexly shaped tissue defects renders them attractive for clinical use. Especially, open
porous microspheres that provide sufficient internal space for cell proliferation and
nutrient diffusions can efficiently aid to completing reconstructions of tissue defects. In
this work, chemically synthesized and biodegradable poly(4-hydroxybutyrate) (P4HB),
which is the U.S. FDA-approved polyhydroxyalkanoate (PHA), was employed for
fabricating open porous microspheres using a double-emulsion solvent evaporation
method. The influences of fabrication parameters were discussed. It was found that the
P4HB-based cell-free and growth factor-free open porous microspheres can enhance
osteoblast differentiation of adipose-derived stem cells in vitro and accelerate rat calvarial
bone-defect healing in vivo. These results demonstrated that the injectable open porous
P4HB microspheres present a remarkable potential in bone tissue regeneration.