Fabrication of hydrogel with cell adhesive micropatterns for mimicking the oriented tumor-associated extracellular matrix
writer：Zhaobin Guo, Ke Hu, Jianfei Sun, Tianzhu Zhang*, Qiying Zhang, Lina Song, Xizhi Zhang, and Ning Gu*
keywords：magnetostatic field induced assembly, magnetic nanomaterials, cell-adhesive micropatterns, agarose hydrogel, tumor-associated ECM
source：期刊
specific source：ACS Applied Materials and Interfaces, 2014, 6: 10963–10968
Issue time：2014年
For mimicking the fibrous extracellular matrix (ECM), a facile method for patterning anticell adhesive substrate was novelly applied on agarose hydrogel. Without using masks or templates for etching, we applied the magnetic field-induced colloidal assembly of magnetic nanoparticles on the flat agarose hydrogel to form cell-adhesive micropatterns.
Meanwhile, tuning the hydrogel substrate’s modulus to fit real tissue was experimentally demonstrated. Magnetic nanobeads were also assembled on this hydrogel surface and formed more complete and regular patterns. The patterned hydrogel substrate could actually influence behaviors of different cancer cells, including adhesion, growth, and migration.