23. pH- and glucose-sensitive ((PAA-co-PAAPBA)-b-)2PAEG nanoparticles for triggered release of insulin.
作者：Yanxia Wang, Xinge Zhang*, Cui Cheng, Chaoxing Li*.
關鍵字：glucose-sensitivity, nanoparticles
論文來源：期刊
具體來源：Carbohydrate Polymers, 2012, 89(1), 124-131.
發表時間：2012年
Amphiphilic poly(acrylic acid-co-acrylamidophenylboronic acid)-block-poly(2-acryloxyethyl galactose)-block-poly(acrylic acid-co-acrylamidophenylboronic acid) (((PAA-co-PAAPBA)-b-)2PAEG) copolymer was fabricated: The poly(2-acryloyloxyethyl pentaacetylgalactoside) (PAEAcG) with narrow molecular weight distributions (Mw/Mn ≤ 1.22) was prepared by atom transfer radical polymerization (ATRP) using dibromo-p-xylene (DBX) as initiator. Then the well-defined triblock copolymer poly(t-butyl acrylate)-b-poly(2-acryloyloxyethyl pentaacetylgalactoside)-b-poly(t-butyl acrylate) (PtBA-b-PAEAcG-b-PtBA) was synthesized by ATRP of tBA using PAEAcG homopolymer with dibromo end groups as macroinitiator. After hydrolysis of t-butyl acrylate block, amide linkage and deacetylation, the final copolymer ((PAA-co-PAAPBA)-b-)2PAEG was obtained. Because of characteristics of three different segments, amphiphilic ((PAA-co-PAAPBA)-b-)2PAEG can self-assemble into pH- and glucose-responsive nanoparticles studied by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Furthermore, the in vitro release profiles of insulin also revealed obvious pH- and glucose-sensitivity of the nanoparticles. The analysis of cell viability suggested that the copolymer nanoparticles had good cytocompatibility. It is the first time that the kinetic for the ATRP of 2-acryloyloxyethyl pentaacetylgalactoside is studied. ao We find that a degree of control for the ATRP of tBA using poly(2-acryloyloxyethyl pentaacetylgalactoside) macroinitiators. ao DLS, TEM and the in vitro release study reveal obvious pH- and glucose-sensitivity of the glycopolymer nanoparticles. ao The sugar blocks improve the cytocompatiblity of the glycopolymer nanoparticles.