Synthesis and Gene Delivery of Poly(amido amine)s with Different Branched Architecture
作者：Wang, R．B.; Zhou, L. Z.; Zhou, Y. F.; Li, G. L.; Zhu, X. Y.*; Gu, H. C.; Jiang, X. L.; Li, H. Q
關鍵字：Gene Delivery, Poly(amido amine)s
論文來源：期刊
具體來源：Biomacromolecules 2010, 11, 489. http://pubs.acs.org/doi/abs/10.1021/bm901215s
發表時間：2010年
A general strategy to improve the transfection efficiency as well as lower the cytotoxicity for polycationic vectors has been developed. Through the polycondensation addition of N,N′-methylene bisacrylamide and 1-(2-aminoethyl)piperazine in a water/N,N-dimethylformamide cosolvent, a series of cationic poly(amido amine)s with same repeating units but different branched architecture have been prepared. With the increase in branched architecture, the cationic polymers become more and more compact, accompanied by the enhancement of primary and tertiary amino groups. Therefore, the buffering capacities and DNA condensation capabilities of cationic poly(amido amine)s are strengthened greatly, whereas the correspondent cytotoxicity decreases. Correspondingly, the transfection efficiency is improved by more than three orders of magnitude. The results of this study indicate that the gene delivery can be readily regulated by only changing the branched architecture of polycations.