綆浠?/strong>
銆銆浜轟滑鐨勫叧娉ㄧ偣鏇村鍦伴泦涓湪寰泭娉″拰澶栨硨浣擄紝鍥犱負瀹冧滑姝ｈ秺鏉ヨ秺澶氱殑琚紩鐢ㄤ負涓涓綔鍦ㄧ殑鐢熺墿鏍囪銆傝櫧鐒跺湪榪欎竴鏂板叴棰嗗煙鍐呯殑瀹氫箟榪樹笉澶熸寮忥紝浣嗚繖涓ょ被鐢熺墿綰崇背棰楃矑閮藉彲浠ラ氳繃鍏剁矑搴﹁寖鍥村拰鐢熺墿璧鋒簮鍔犱互鍖哄垎銆?閫氬父錛屽井娉＄殑鐩村緞涓?00 nm鑷? 渭m錛岃屽娉屼綋鐨勭洿寰勪負30 nm - 100 nm銆?寰場涓鑸槸閫氳繃緇嗚優璐ㄨ啘璧鋒場褰㈡垚鐨勶紝鑰屽娉屼綋鍒欓氳繃鏍稿唴浣撶殑澶氭場浣撹優鍚愪綔鐢ㄩ噴鏀懼嚭鏉ャ?涓よ呬技涔庡潎鍙備笌緇嗚優淇″彿浼犲錛屽彲鎼哄甫涓緋誨垪淇″彿浼犲铔嬬櫧浠ュ強淇′嬌RNA鍜屽井microRNA鍒嗗瓙 涓よ呭湪琛娑蹭腑鐨勫驚鐜按騫沖湪浼楀鐤劇梾涓兘琛ㄧ幇涓哄楂橈紝鍏朵腑鍖呮嫭鍔ㄨ剦綺ユ牱紜寲鍜屽啝鐘跺姩鑴夌柧鐥呫佽娑茬梾鍜岀値鐥囨х柧鐥呫佺硸灝跨梾浠ュ強鐧岀棁銆?
銆銆鐩墠錛屽娉屼綋鐨勭爺絀朵竴鐩村洜緙轟箯鍚堥傜殑琛ㄥ緛嫻嬭瘯鏂規硶鑰屽彈鍒伴檺鍒躲?椹皵鏂囩殑NanoSight緋誨垪浜у搧閲囩敤鐙竴鏃犱簩鐨勬垚鐔熸妧鏈屼嬌榪欎竴闇姹傚緱鍒版弧瓚熾?綰崇背棰楃矑璺熻釜鍒嗘瀽錛圢TA錛夋妧鏈厑璁稿疄鏃跺湴瀵規偓嫻恫涓?0 nm - 1000 nm鐩村緞鑼冨洿鍐呯壒瀹氱殑澶栨硨浣撳拰寰泭娉¤繘琛岄愪釜鐨勭洿鎺ユ垚鍍忓拰瑙傚療銆?鍚屾椂錛孨TA鍙彁渚涢珮鍒嗚鯨鐜囩殑綺掑害鍒嗗竷鍥捐〃鍜屾祿搴︿俊鎭?璇ユ妧鏈槗鐢ㄣ佸揩閫熴佺ǔ鍋ャ佸噯紜笖浣跨敤鎴愭湰浣庯紝鏄鐜版湁鏂規硶鐨勪竴涓壇濂借ˉ鍏呫?鍦ㄨ崸鍏夋ā寮忎笅閫氳繃涓緋誨垪涓嶅悓嬋鍙戞嘗闀跨殑嬋鍏夛紝鍙互瀵規牱鏈腑鐨勫凡緇忚鏍囪鐨勯綺掕繘琛岃〃寰佺殑嫻嬪畾鍜岄壌鍒?
媯嫻嬪拰嫻嬪畾緇嗚優澶栧泭娉＄殑嫻撳害
銆銆鍔ㄦ佸厜鏁ｅ皠錛圖LS錛夊拰NTA閮藉彲浠ラ氳繃鏂墭鍏嬫柉-鐖卞洜鏂潶鏂圭▼寮忔祴瀹氳繍鍔ㄩ熷害鎴栨墿鏁ｇ郴鏁幫紙Dt錛変笌綺掑害鏈夊叧鐨勭撼綾抽綺掔殑甯冩湕榪愬姩銆?
銆銆浣跨敤NTA鏃訛紝嬋鍏夌収灝勬偓嫻恫涓殑棰楃矑錛屽茍鐢ㄨ棰戞憚鍍忔満鎹曡幏鎵浜х敓鐨勬暎灝勫厜銆?閫氳繃璺熻釜鍗曚釜棰楃矑鍦ㄤ簩緇村鉤闈笂鐨勪綅緗彉鍖栨潵紜畾棰楃矑鐨勬墿鏁ｆ儏鍐點?濡傛灉鎵╂暎緋繪暟錛圖t錛夊凡鐭ワ紝灝卞彲浠ョ‘瀹氶綺掔殑嫻佷綋鍔涘鐩村緞銆?
銆銆涓嬪浘鏄劇ず鍑洪綺掑湪婧舵恫涓繘琛屽竷鏈楄繍鍔ㄧ殑鎯呭喌銆?鍒濇鐩媯鏌ヨ〃鏄庯紝瀛樺湪杈冨ぇ棰楃矑鎴栬仛闆嗙墿錛堝浘1A錛夈?鐒跺悗錛孨TA杞歡蹇熺敓鎴愰愪釜棰楃矑鐨勯珮鍒嗚鯨鐜囩矑搴﹀垎甯冧互鍙婃墍瑙傚療鍒板泭娉￠綺掔殑璁℃暟錛堜緷鎹粷瀵硅鏁版祿搴︼級錛堝浘1B錛夈?
鍥?* 紼閲婄殑鏃犺灝忔澘琛嫻嗘牱鍝?A錛塏anoSight鎶鏈敓鎴愮殑鍏稿瀷鍥懼儚 鎵鐢熸垚鐨勫浘鍍忚鐢ㄦ埛鑳藉绔嬪嵆璇嗗埆鍏舵牱鍝佺殑鏌愪簺鐗規э紙鍖呮嫭嫻撳害鍜屽鍒嗘暎鎬ф按騫籌級銆?B錛夋牱鍝佺殑綺掑害鍒嗗竷涓庡師濮嬫祿搴﹁綆楀?*緇撴灉鏉ユ簮錛欳 Gardiner1, RDragovic1, A Brooks1, D Tannetta1, D Redman2, P Harrison2, and I Sargent1錛?010錛?Nanoparticle TrackingAnalysis for the Measurement and Characterisation of Cellular Microvesicles and Nanovesicles, Proc NVTH BSTH 2010.
1Nuffield Department of Obstetrics and Gynaecology, University of Oxford
2Oxford Haemophilia & Thrombosis Centre, Churchill Hospital , Oxford, UK
鏇夸唬鎶鏈?/strong>
浼犵粺涓婏紝璁稿鎶鏈凡琚敤鏉ヨ〃寰佸井綾沖拰綰崇背鍥婃場錛屽悓鏃舵湁鏇村鎶鏈彲鐢ㄦ潵鍒嗘瀽寰瀷棰楃矑銆?鍏朵腑鍖呮嫭錛?
路 嫻佸紡緇嗚優璁℃暟娉?
路 鍔ㄦ佸厜鏁ｅ皠錛圖LS錛?
路 鐢靛瓙鏄懼井闀滄鏌ユ硶錛圗M錛?
路 閰惰仈鍏嶇柅鍚擱檮鍒嗘瀽錛圗LISA錛?
銆銆涓婅堪鎶鏈腑搴旂敤鏈騫挎硾鐨勬槸嫻佸紡緇嗚優璁℃暟娉曘傞氬父錛屽晢鐢ㄦ祦寮忕粏鑳炰華鐨勫疄闄呯矑搴︿笅闄愮害涓?00 nm錛堝浜庤仛鑻箼鐑綺掞級錛涘湪璇ユ暟鍊間笅錛屼俊鍙鋒棤娉曚笌鍩虹嚎鍣煶鍖哄垎寮鏉ャ傝櫧鐒墮氳繃浣跨敤鑽у厜鏍囪鍙互鎵╁睍璇ユ嫻嬮檺鍊鹼紝浣嗙矑搴﹁緝灝忔椂錛屽噯紜祴瀹氳繖綾婚綺掑ぇ灝忕殑鑳藉姏涓ラ噸鍙楅檺銆?DLS涔熷凡鐢ㄤ簬姝ら」搴旂敤錛屽茍鍙負鍗曞垎鏁ｆ牱鍝佹彁渚涚簿紜殑綺掑害淇℃伅銆?NTA鍙互涓洪渶瑕侀珮鍒嗚鯨鐜囩矑搴﹀垎甯冪殑DLS鎻愪緵鍏朵粬姝ｄ氦淇℃伅銆?鐢靛瓙鏄懼井闀滄鏌ユ硶鏄竴縐嶉潪甯告湁鐢ㄧ殑寰背鍜岀撼綾沖泭娉＄爺絀跺伐鍏鳳紝浣嗗叾浠ｄ環鏄祫閲戣繍琛屾垚鏈珮銆佹牱鍝佸埗澶囨槀璐點佸鐞嗘椂闂撮暱浠ュ強鏍峰搧鍒跺鍚庣殑鏍峰搧瀹屾暣鎬у樊銆?
嫻嬪畾欏圭洰鐨勫彲閫夋嫨鎬?/strong>
銆銆铏界劧瀹冮氬父瓚充互紜畾鏍峰搧涓槸鍚﹀瓨鍦ㄦ煇涓綺掑害鎴栫矑搴﹁寖鍥寸殑棰楃矑錛屼絾瀹冭繕鍏鋒湁閴村埆鍜屽尯鍒嗘牱鍝佸唴鐗瑰畾鐨勪簹緹よ惤棰楃矑鐨勯檮鍔犱環鍊箋侼anoSight鎶鏈兘澶熷熷姪綾諱技鎶椾綋浠嬪鍨嬭崸鍏夋爣璁扮瓑鏂規硶錛岄夋嫨鎬у湴鍒嗘瀽榪欑被緹よ惤銆?榪欑鏂規硶浣垮緱鐢ㄦ埛鍙互鍙夋嫨媯嫻嬪拰鍒嗘瀽緇撳悎鏈夎崸鍏夋爣璁板瀷鎶椾綋鐨勭壒寮傛х撼綾抽綺掞紝鑰岄潪鐗瑰紓鎬ц儗鏅綺掑垯閫氳繃浣跨敤閫傚綋鐨勬護鍏夊櫒鑰岃鎺掗櫎銆傝櫧鐒跺彲浠ヤ嬌鐢ㄤ竴緋誨垪鑽у厜鍥紝浣嗕嬌鐢ㄩ珮鏁堛侀珮紼沖畾鎬х殑閲忓瓙鐐規爣璁版洿鍏蜂紭鍔匡紝鍙互鍙栧緱鏈浣蟲晥鏋溿?
銆銆璇ユ妧鏈鍥?A鎵紺猴紝閫氳繃瑁呮湁钃濈傳鑹叉縺鍏変簩鏋佺錛?05 nm錛夌殑NanoSight 浠櫒嬋鍙戠殑閲忓瓙鐐圭殑涓涓棰戝撫鐨勮崸鍏変俊鍙楓?榪欎簺閲忓瓙鐐圭敤鏉ユ爣璁板鍚堣優浣撴粙鍏誨眰寰場錛圫TBM錛変笂鐨勭洰鏍囩敓鐗╂爣璁板叿鏈夌壒寮傛х殑涓縐嶆姉浣擄紙NDOG II錛夈?
銆銆鍥?B鏄劇ず鍑轟笁縐嶇矑搴﹀垎甯冿紝鍏朵腑鍖呮嫭錛?i錛夛紙闈炶崸鍏夛級鍏夋暎灝勫櫒媯嫻嬪埌鐨凷TBM鏍峰搧涓殑鎵鏈夐綺掞紙钃濈嚎錛夛紱 ii錛夊湪鑽у厜妯″紡涓嬫祴瀹氱殑緇撳悎鏈夎崸鍏夐噺瀛愮偣鏍囪 NDOG II鎶椾綋鐨勯綺掞紙綰㈢嚎錛夛紱鍜?iii錛変竴縐嶅鐓у搧錛堢豢綰匡級錛岀敱涓涓被浼肩殑閲忓瓙鐐規爣璁版姉浣撶粍鎴愶紝浣嗘姉浣撳STBM涓婄殑鐩爣鐢熺墿鏍囪娌℃湁浜插拰鍔涳紙鍚屾牱鍦ㄨ崸鍏夋ā寮忎笅嫻嬪畾錛夈?榪欒鏄庯紝鏍峰搧涓殑澶у鏁伴綺掗兘鎴愬姛浣跨敤STBM鐗瑰紓鎬ч噺瀛愮偣NDOG II鎶椾綋榪涜浜嗙壒寮傛ф爣璁幫紝騫朵笖瀵圭収鍝佷篃鎴愬姛鏄劇ず鍑鴻秴浣庣殑鑳屾櫙淇″彿銆?
鍥?* A錛?鍊熷姪鎶椾綋涓嶴TBM棰楃矑鐩哥粨鍚堢殑閲忓瓙鐐規墍寰楀嚭鐨勮崸鍏夊浘鍍?B錛?鏉ヨ嚜鏁ｅ皠鍏夛紙钃濊壊錛夈佹紜姉浣擄紙綰㈣壊錛夊拰闈炴紜紙瀵圭収錛夋姉浣擄紙緇胯壊錛夌殑綺掑害鍒嗗竷 璇鋒敞鎰忚鏁版祿搴︾旱杞淬?/span>
緇撹
銆銆NTA鑳藉瀵逛綆嫻撳害鐨勫井鍥婃場鍜屽娉屼綋榪涜澶у皬鍜屾祿搴︽祴瀹氾紝騫朵笖鍦ㄤ笌鑽у厜鏍囪鑱斿悎浣跨敤鏃訛紝鍙互閫夋嫨鎬у湴紜畾鍜屽垎鏋愬鏉傛牱鍝佷腑鐗瑰畾綾誨瀷鐨勯綺掋?/span>
