縐戝瀹跺湪媧葷粏鑳炶秴鍒嗚鯨鐜囨垚鍍忛鍩熷彇寰楅噸瑕佽繘灞?/span>
2015-8-31 鏉ユ簮:涓浗鑱氬悎鐗╃綉 鐐瑰嚮嬈?/span>
鍏抽敭璇嶏細鏄懼井闀?/a> 瓚呭垎杈ㄧ巼 鎴愬儚
鏉ヨ嚜緹庡浗闇嶅崕寰蜂紤鏂尰瀛︾爺絀舵墍Janelia鐮旂┒鍥佷腑鍥界瀛﹂櫌鐢熺墿鐗╃悊鐮旂┒鎵銆佺編鍥藉浗绔嬬瀛︾爺絀墮櫌銆佸搱浣涘尰瀛﹂櫌絳夌殑縐戝瀹朵滑錛屽熷姪鍏跺彂灞曠殑鏂板厜瀛﹁秴鍒嗚鯨鐜囨垚鍍忔妧鏈紝鍦ㄥ墠鎵鏈湁鐨勯珮鍒嗚鯨鐜囨潯浠朵笅鐮旂┒浜嗘椿浣撶粏鑳炲唴鐨勫姩鎬佺敓鐗╄繃紼嬨備粬浠殑鏂版柟娉曟樉钁楁彁楂樹簡緇撴瀯鍏夌収鏄庢樉寰暅(structured illumination microscopy, SIM)鐨勫垎杈ㄧ巼錛屼竴縐嶆渶閫傚悎媧諱綋瓚呭垎杈ㄦ垚鍍忕殑鎶鏈?/span>
鏂版妧鏈墍鎷嶆憚鐨勮棰戠敓鍔ㄥ湴灞曠幇浜嗙粏鑳炲唴铔嬬櫧璐ㄧ殑榪愬姩鍜岀浉浜掍綔鐢ㄣ傚畠浠府鍔╃敓鐗╁瀹剁悊瑙ｇ粏鑳炴槸鎬庢牱鏀瑰彉瀹冧滑涔嬮棿鐨勪緷瀛樼粨鏋勶紝浠ュ強閲嶆暣緇嗚優鑶滅粨鏋勪嬌寰楃粏鑳炲鐨勫垎瀛愬彲浠ヨ鍚告敹鍒扮粏鑳炲唴銆傛潵鑷狫anelia鐮旂┒鍥殑鐮旂┒鍛楨ric Betzig銆佺敓鐗╃墿鐞嗘墍鏂板紩榪汸I鏉庢爧鍜屼粬浠殑鍚堜綔鑰呭熀浜庡師鏈夌殑SIM鏄懼井闀滃師鐞嗘柊鍙戝睍浜嗕袱縐嶆柊鐨勮秴鍒嗚鯨鐜囨垚鍍忔妧鏈傝秴鍒嗚鯨鐜囧厜瀛︽樉寰垚鍍忔妧鏈兘澶熻法瓚婄悊璁虹殑鍒嗚鯨鐜囨瀬闄愶紝鍦ㄦ瀬楂樼殑鍒嗚鯨鐜囦笅灞曠幇緇嗚優鍐呯殑綺劇粏緇撴瀯銆備絾鏄紝鍒扮洰鍓嶄負姝紝瓚呭垎杈ㄧ巼鏄懼井闀滄妧鏈嵈渚濈劧涓嶈兘榪涜鏈夋晥鐨勬椿浣撶粏鑳炴垚鍍忋?/span>
鈥滆繖浜涙柟娉曡绔嬩簡瓚呭垎杈ㄧ巼鍏夊鏄懼井闀滅殑鎴愬儚閫熷害鍜岄潪渚靛叆鐗規х殑鏂版爣鍑嗭紝瀹冧滑浣垮緱瓚呭垎杈ㄧ巼媧諱綋緇嗚優鎴愬儚鎴愪負鐜板疄銆傗滲etzig璇撮亾銆傝繖涓鐮旂┒鎴愭灉浜?鏈?8鏃ュ湪緹庡浗銆婄瀛︺嬫潅蹇椾笂浠ュ皝闈㈡枃绔犲彂琛ㄣ?/span>
鍦ㄤ紶緇熺殑SIM鏄懼井闀滀腑錛岀墿闀滀笅鐨勭墿浣撹闈炲潎鍖鐨勭粨鏋勫厜錛堢被浼間簬鏉＄汗鐮侊級鎵鐓ф槑銆傚湪瀹為獙涓紝鍑犳潫涓嶅悓鐨勭粨鏋勫厜鐢ㄦ潵鐓ф槑鐗╀綋錛屽畠浠拰鐗╀綋鍦ㄤ笉鍚岃搴︽販棰戞墍浜х敓鐨勬懇灝旀潯綰硅鐩告満渚濇閲囬泦銆傜劧鍚庤綆楁満鎻愬彇鎽╁皵鏉＄汗緙栫爜鐨勪俊鎭茍灝嗗叾瑙ｇ爜鐢熸垚涓夌淮鐨勯珮鍒嗚鯨鐜囧浘鍍忋傛渶緇堥噸寤虹殑SIM鍥懼儚鍏鋒湁楂樹簬浼犵粺鏄懼井闀滃浘鍍?鍊嶇殑絀洪棿鍒嗚鯨鐜囥?/span>
Betzig鍜屽叾浠栦袱浣嶇瀛﹀鍥犱負鍙戝睍瓚呭垎杈ㄧ巼鑽у厜鏄懼井闀滆岃鎺堜簣2014騫磋璐濆皵鍖栧濂栥備粬璇撮亾錛孲IM鏄懼井闀滄妧鏈箣鎵浠ユ病鏈夊緱鍒板儚鍏跺畠鏂規硶閭ｆ牱澶氱殑鍏蟲敞錛屾槸鍥犱負鍏跺畠鎶鏈兘澶熸彁渚涙瘮涓ゅ嶆洿楂樼殑鍒嗚鯨鐜囨敼榪涙晥鏋溿備絾鏄紝浠栧己璋僑IM鎷ユ湁涓ゅぇ鍏跺畠鐨勮秴鍒嗚鯨鐜囨柟娉曟墍娌℃湁鐨勪紭鍔褲傝繖浜涘叾瀹冩柟娉曞寘鎷簡涓ょ鍘誨勾鑾峰緱璇鴻礉灝斿琛ㄥ槳鐨勬妧鏈細浠栧拰鍚屼簨Harald Hess浜?006騫村紑鍙戠殑鍏夋縺媧誨畾浣嶆樉寰暅錛坧hotoactivated localization microscopy, PALM錛夛紝鍜屽彈嬋杈愬皠鑰楀敖錛坰timulated emission depletion錛孲TED錛夋樉寰暅銆備絾鏄紝榪欎袱縐嶆妧鏈兘闇瑕佽繃澶氭垨榪囧己鐨勫厜鏉ョ収鏄庢牱鍝侊紝浠ヨ嚦浜庤崸鍏夎泲鐧藉緢蹇婕傜櫧錛岀粏鑳炴牱鍝佸緢蹇鎹熷錛屼粠鑰屼笉鍙兘闀挎椂闂磋繘琛屾垚鍍忋傜劧鑰岋紝SIM鍦ㄨ繖浜涙柟闈笉涓鏍鳳紝鈥滄垜鐖變笂浜哠IM錛屽洜涓哄畠鐨勯熷害寰堝揩錛岃屼笖瀹冩墍闇鐨勭収鏄庡厜寮哄害榪滆繙灝忎簬鍏跺畠鏂規硶銆傗滲etzig璇撮亾銆?/span>
Betzig鍦?011騫碝ats Gustafsson鍘諱笘鍚庝笉涔呭紑濮嬩笌SIM鐩稿叧鐨勭爺絀躲侴ustafsson鏄疭IM鎶鏈殑鍏堥┍涔嬩竴錛岀敓鍓嶄篃鏄疛anelia鐨勭爺絀跺憳銆侭etzig閭ｆ椂宸茬粡娣變俊SIM鏈夋綔鍔涗負瑙ｆ瀽緇嗚優鍐呴儴鐨勫伐浣滄満鐞嗘彁渚涢噸瑕佺殑瑙佽В錛屽鏋淪IM鐨勭┖闂村垎杈ㄧ巼鍙互琚彁楂橈紝瀹冨浜庣敓鐗╃爺絀剁殑鍙敤鎬у皢琚ぇ澶у寮恒?/span>
鍦ㄧ敓鍓嶏紝Gustafsson鍜屽崥澹敓Hesper Rego鍙戝睍浜嗕竴縐嶅埄鐢ㄩケ鍜岃楀敖錛坰aturated depletion錛夌殑闈炵嚎鎬IM鎶鏈紝浣嗚繖縐嶆妧鏈湪鏀硅繘鍒嗚鯨鐜囩殑鍚屾椂闇瑕佷嬌鐢ㄥ緢澶氱殑鍏夌収騫朵笖鏁ｅけ浜哠IM鎴愬儚閫熷害蹇殑浼樺娍銆侭etzig鎯沖埌浜嗕竴縐嶅彲浠ラ伩鍏嶈繖浜涚己闄風殑鏂規硶銆?/span>
楗卞拰鑰楀敖闈炵嚎鎬IM鍒╃敤鍏夊彲鍙嶅寮鍏崇殑鑽у厜铔嬬櫧鍜屽叾鍦ㄥ紑鍏寵繃紼嬩腑鐨勯ケ鍜岃楀敖鏁堝簲鏉ユ彁楂樺垎杈ㄧ巼銆傚畠浜х敓鍥懼儚鐨勮繃紼嬫槸錛岄鍏堟妸鎵鏈夌殑鑽у厜铔嬬櫧鍒嗗瓙嬋媧誨埌鍙彂鍏夌殑鐘舵侊紙浜侊級錛岀劧鍚庣敤涓鏉熺粨鏋勫厜鎶婂ぇ閮ㄤ喚鐨勪寒鎬佸垎瀛愬弽嬋媧誨埌鏆楁併傞氳繃緇撴瀯鍏夊弽嬋媧諱箣鍚庯紝浠呮湁灝戞暟澶勪簬緇撴瀯鍏夋渶寮卞尯鍩熺殑鍒嗗瓙浠嶇劧淇濇寔鍦ㄤ寒鎬併傝繖浜涘厜璋冩帶榪囩▼鎻愪緵浜嗙墿浣撶殑楂樼┖闂撮鐜囦俊鎭紝浠庤岃鍥懼儚鏇村姞娓呮櫚銆傝繖涓榪囩▼闇瑕侀噸澶?5鎴栨洿澶氭鎵嶈兘浜х敓鏈緇堢殑楂樺垎杈ㄧ巼鍥懼儚銆侭etzig璇撮亾錛岃繖涓鍘熺悊闈炲父綾諱技浜嶴TED鎴栧彟涓縐嶄笌鍏剁浉鍏崇殑鍙仛RESOLFT鐨勮秴鍒嗚鯨鐜囨妧鏈殑鍘熺悊銆?/span>
榪欎竴鎶鏈茍涓嶉傚悎浜庢椿浣撴垚鍍忥紝鍥犱負嬋媧誨拰鍙嶆縺媧昏崸鍏夎泲鐧介渶瑕佸緢闀跨殑鏃墮棿銆傚彟澶栵紝鍙嶅鐨勫厜鐓ф槑浼氬緇嗚優鍜岃崸鍏夎泲鐧芥湰韜犳垚鎹熶激銆侭etzig璇撮亾錛屸滆繖涓鎶鏈殑闂鍦ㄤ簬浣犻鍏堢敤鍏夋縺媧諱簡鎵鏈夌殑鑽у厜铔嬬櫧鍒嗗瓙錛岀劧鍚庝綘椹笂鍙堢敤鍙︿竴鏉熷厜鍙嶆縺媧諱簡澶ч儴浠藉垎瀛愩傝繖浜涜鍙嶆縺媧葷殑鍒嗗瓙瀵規渶緇堢殑鍥懼儚娌℃湁浠諱綍璐＄尞錛屼絾鍗磋浣犵敤鍏夆樻補鐐糕欎簡涓ゆ銆備綘璁╁垎瀛愭壙鍙椾簡寰堝ぇ鈥樺帇鍔涒欙紝騫朵笖鑺變簡寰堝浣犲茍娌℃湁鐨勬椂闂達紝鍥犱負榪欐鏃墮棿鍐呯粏鑳炲湪榪愬姩銆傗?/span>
瑙ｅ喅鏂規硶鍏跺疄寰堢畝鍗曪紝Betzig璇撮亾錛氣滄病鏈夊繀瑕佹縺媧繪墍鏈夌殑鍒嗗瓙銆傗濆湪Betzig鐮旂┒灝忕粍鏂板彂灞曠殑緇撴瀯鍏夋縺媧婚潪綰挎IM鐨勬妧鏈腑錛屼竴寮濮嬬敤緇撴瀯鍏夊彧嬋媧繪牱鍝侀噷鐨勪竴閮ㄥ垎鑽у厜铔嬬櫧鍒嗗瓙銆傗滆繖涓緇撴瀯鍏夋縺媧昏繃紼嬪凡緇忕粰浣犱竴浜涢珮鍒嗚鯨鐜囩殑淇℃伅浜嗐傗滲etzig瑙ｉ噴閬撱傚彟澶栦竴鏉熺粨鏋勫厜鐢ㄤ簬鍙嶆縺媧誨垎瀛愶紝棰濆鐨勪俊鎭彲浠ュ湪鍙嶆縺媧葷殑榪囩▼涓悓鏃惰璇誨嚭銆備袱涓粨鏋勫厜鍙犲姞鐨勬晥搴旂粰涓庢渶緇堝浘鍍?2綰崇背鐨勫垎杈ㄧ巼錛岃繖涓緇撴灉濂戒簬鍘熷鐨凷IM錛屽茍涓旀妸鐢卞厜娉㈤暱鍐沖畾鐨勪紶緇熷垎杈ㄧ巼鏋侀檺鏀硅繘浜嗕笁鍊嶃?/span>
鈥滄垜浠兘澶熷仛鍒板揩閫熷湴瓚呴珮鍒嗚鯨鐜囨垚鍍忋傗滲etzig璇撮亾銆傝繖寰堥噸瑕侊紝浠栬ˉ鍏呴亾錛屽洜涓哄浜庡姩鎬佽繃紼嬶紝鍗曠函鎻愰珮絀洪棿鍒嗚鯨鐜囪屾病鏈夌浉搴斿湴鎻愰珮鎴愬儚閫熷害鏄病鏈夋剰涔夌殑銆傗滃鏋滅粏鑳炲唴閮ㄦ湁鐨勭粨鏋勪互1寰背姣忕鐨勯熷害榪愬姩錛屽茍涓旀垜鏈?寰背鐨勫垎杈ㄧ巼錛岄偅涔堟垜闇瑕佸湪涓縐掑唴閲囬泦鍥懼儚銆備絾濡傛灉鎴戞湁1/10寰背鐨勫垎杈ㄧ巼錛岄偅涔堟垜灝卞繀闇鍦?/10縐掑唴閲囬泦鍥懼儚錛屼笉鐒跺浘鍍忓皢鍙樺緱妯＄硦銆傗滲etzig瑙ｉ噴閬撱?/span>
緇撴瀯鍏夋縺媧婚潪綰挎IM鍙湪1/3縐掑唴閲囬泦25騫呭師濮嬪浘鍍忥紝騫朵粠涓噸寤哄嚭涓騫呴珮鍒嗚鯨鐜囧浘鍍忋傚畠鐨勫浘鍍忛噰闆嗗緢楂樻晥錛屽彧闇鐢ㄨ緝浣庣殑鐓ф槑鍏夊己錛屽茍涓旀敹闆嗘瘡涓涓寒鎬佽崸鍏夎泲鐧藉垎瀛愭墍鎼哄甫鐨勪俊鎭備粠鑰屾湁鏁堝湴淇濇姢浜嗚崸鍏夊垎瀛愶紝浣垮緱鏄懼井闀滆兘澶熻繘琛屾洿闀挎椂闂寸殑鎴愬儚錛岃縐戝瀹朵滑鍙互瑙傛祴鍒版洿澶氱殑鍔ㄦ佹椿鍔ㄣ?/span>
璇ユ垚鍍忔妧鏈紙PA NL-SIM錛夌殑瀹炵幇闇瑕佷竴綾昏兘琚弽澶嶅厜嬋媧葷殑鑽у厜铔嬬櫧銆傚悎浣滆呯敓鐗╃墿鐞嗘墍寰愬鉤鍕囪棰樼粍鍙戝睍浜嗕竴縐嶆柊鍨嬪弽澶嶅厜嬋媧昏崸鍏夎泲鐧絊kylan-NS錛屽姣旂幇鏈夌殑鍏跺畠鍙嶅鍏夋縺媧葷殑鑽у厜铔嬬櫧錛岃铔嬬櫧鍏鋒湁楂樺姣斿害銆侀珮鍏夊紼沖畾鎬х瓑鐗規с傗淪kylan-NS鏄捐憲鏀硅繘浜嗚繖浜涘闈炵嚎鎬IM鏉ヨ鑷沖叧閲嶈鐨勭壒鎬э紝浣垮緱鎴戜滑鍦ㄦ妧鏈柟闈㈢殑鍒涙柊鑳藉浣撶幇寰楁穻婕撳敖鑷淬傚洖欏捐秴鍒嗚鯨鐜囨樉寰暅鐨勫彂鏄庡拰鍙戝睍榪囩▼錛屾柊棰栫殑鑽у厜鎺㈤拡涓鐩存壆婕斿叧閿鑹詫紝鍦ㄨ繖涓宸ヤ綔涓篃涓嶄緥澶栥傗濇潕鏍嬭閬撱?/span>
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閫氳繃楂樻暟鍊煎瓟寰勭殑鏂規硶錛孊etzig鐨勫洟闃熻嫻嬩簡澶氫釜楠ㄦ灦铔嬬櫧璐ㄥ湪褰㈡垚綺樼潃鏂戯紙閾炬帴緇嗚優鍐呭鐨勭墿鐞嗛摼錛夎繃紼嬩腑鐨勮繍鍔ㄥ拰鐩鎬簰浣滅敤銆備粬浠篃榪借釜浜哻lathrin淇グ鐨勫唴鍚炰綋鐨勬垚闀垮拰鍐呭悶榪囩▼錛堝唴鍚炰綋灝嗙粏鑳炲鐨勫垎瀛愯漿縐誨埌緇嗚優鍐咃級銆備粬浠殑瀹氶噺鍒嗘瀽鍥炵瓟浜嗗嚑涓笉鑳借浠ュ線鐨勬垚鍍忔妧鏈墍瑙ｅ喅鐨勯棶棰橈紝渚嬪錛屽唴鍚炰綋鐨勫垎甯冿紝浠ュ強鍐呭悶浣撳昂瀵稿拰瀵垮懡涔嬮棿鐨勫叧緋匯傛渶鍚庯紝閫氳繃緇撳悎楂樻暟鍊煎瓟寰勬柟娉曞拰緇撴瀯鍏夋縺媧婚潪綰挎IM錛孊etzig鍜屼粬鐨勫悓浜嬪彲浠ュ湪瓚呴珮鍒嗚鯨鐜囨潯浠跺悓鏃惰拷韙袱縐嶈泲鐧借川鐨勬椿鍔ㄣ?/span>
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璇ュ伐浣滃彈鍒板浗瀹垛?73鈥濊鍒掋佸浗瀹惰嚜鐒剁瀛﹀熀閲戙佸寳浜競鑷劧縐戝鍩洪噾絳夌殑璧勫姪銆?/span>
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娉細鏈綉杞澆鍐呭鍧囨敞鏄庡嚭澶勶紝杞澆鏄嚭浜庝紶閫掓洿澶氫俊鎭箣鐩殑錛屽茍涓嶆剰鍛崇潃璧炲悓鍏惰鐐規垨璇佸疄鍏跺唴瀹圭殑鐪熷疄鎬с?/div>
錛堣矗浠葷紪杈戯細xu錛?
