A polymeric platform for immunodiagnostic bioassay was constructed based on biostable polymeric support andtwo functional monomers, low-fouling methacryloyloxyethyl phosphorylcholine (MPC) and acrylic acid (AAc),by a photografting polymerization approach. Covalent binding of antibody to pAAc chains was achieved by activatingcarboxyl with NHS/EDC partner. The resultant surface showed obvious suppression of nonspecific proteinadsorption and platelet adhesion relative to the control sample, exhibiting good anti-bioadhesion performances.Based on the polymer-supported matrix, a highly sensitive antibody–antigen specific recognitionwas confirmedin both native plasma and diluted human plasma due to the enhanced antibody loading capacity and loweredbioadhesion as compared to the reference.
