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  �@헹���ͨ�^�c�����W�������������������ϳ��Ԛ����� (Cs) ����朡�������-����֦����� (polyamidoamine, PAMAM) ��Ԫ��Ó��đ�� (DCA) ���朵ă��H�����ξۺ��� (PAMAM-Cs-DCA) (Scheme 1)��¹H NMR��FTIR�����_���ˮa��ĽY�� (Figure 1)��

Scheme 1. (A) Schematic representation of co-delivering hydrophobic drug and pDNA by PAMAM-Cs-DCA. Synthesis routes of (B) 6-N₃-Cs and (C) PAMAM-Cs-DCA.

Figure 1. (a) FT-IR spectra of propargyl focal point PAMAM dendron (G=3), 6-N₃-Cs-DCA and PAMAM-Cs-DCA. (b) ¹H NMR spectra of PAMAM dendron (G=3), 6-N₃-Cs-DCA₇, and PAMAM₁₄-Cs-DCA₇ in DMSO-d₆.

  PAMAM-Cs-DCA��ˮ���ԽM�b�γ���PAMAM��Cs���Hˮ�⚤��DCA����ˮ�Ⱥ˵ļ{���z����ͨ�^�ɹ���V���ӑB��ɢ��(DLS)��TEM�yԇ���о���PAMAM-Cs-DCA���z�����О� (Figure 2)��

Figure 2. Intensity ratios (I₁/I₃) from pyrene emission spectra in PBS buffer (pH 7.4) against the logarithm of concentration of (a) PAMAM₁₂-Cs-DCA₁₁ NPs or (b) PAMAM₁₄-Cs-DCA₇. TEM images and size distributions obtained from DLS of the NPs from (c) ) PAMAM₁₂-Cs-DCA₁₁ and (d) PAMAM₁₂-Cs-DCA₁₁-DOX.

  �԰�ù�؞�ģ��ˎ�������PAMAM-Cs-DCA�{���z����ˎ��ؓ�d������ˎ��ጷ��О飬ˎ��ؓ�d���S��DCAȡ���ȵ����Ӷ����ӣ�������ˎ��ጷ��^�����dˎ�z��PAMAM-Cs-DCA-DOX��ˎ��ጷ����ʶ����־��������������S��pHֵ�Ľ��Ͷ�׃�� (Figure 3)��

Figure 3. In vitro release profiles of entrapped DOX from PAMAM₁₂-Cs-DCA₁₁ NPs and PAMAM₁₄-Cs-DCA₇ NPs in phosphate buffer (pH 7.4 or 5.2) at 37 ��. The inset is an enlarged image for showing the initial release. (n=3, mean �� standard deviation)

  ���z�Ӿ��������N/Pֵ���ڵ���1:1�r��PAMAM-Cs-DCA��ؓ�dˎ���PAMAM-Cs-DCA-DOX����ͨ�^�Hˮ��PAMAM��Ԫ����pDNA������ȫ���pDNA�����z�Ӿ�е��w�ơ�DLS��Zeta�λ�yԇ������PAMAM-Cs-DCA��PAMAM-Cs-DCA-DOX�ܺ�pDNA�γ�������200-300 nm�����Ώͺ������늺ɞ���ֵ��MTT�yԇ����PAMAM-Cs-DCA��PAMAM-Cs-DCA-DOX��293T�����о����^�͵ļ������ԣ��h����PEI(25 KDa)�ļ������ԣ�ؓ�dˎ���PAMAM-Cs-DCA-DOX�ļ��������Դ���PAMAM-Cs-DCA��PAMAM-Cs-DCA / pDNA�ͺ����PAMAM-Cs-DCA-DOX / pDNA�ͺ��ﶼ����Ч�،�pDNA�D�M293T�����У��DȾЧ���SN/Pֵ�����Ӷ����ӣ�����ؓ�d��ù�غ󣬏ͺ�����DȾЧ����΢���� (Figure 4��Figure 5)��

Figure 4. Transfection efficiency of PAMAM₁₂-Cs-DCA₁₁ NPs/pDNA, PAMAM₁₄-Cs-DCA₇ NPs/pDNA, PAMAM₁₂-Cs-DCA₁₁-DOX NPs/pDNA and PAMAM₁₄-Cs-DCA₇ -DOX NPs/pDNA complexes in 293T cells at various N/P ratios using PEI/DNA complexes as the control. (*p<0.05, n=3, mean �� standard deviation)

Figure 5. Representative fluorescence images of 293T cells transfected by PAMAM₁₂-Cs-DCA₁₁NPs/pDNA complex (a: N/P 5:1; b: N/P 10:1; c: N/P 20:1), PAMAM₁₂-Cs-DCA₁₁-DOX NPs/pDNA complex (d: N/P 5:1; e: N/P 10:1; f: N/P 20:1), PEI/pDNA complex (g: N/P 10) and native pDNA (h) (AI and BI: fluorescence field images; AII and BII: bright field images).

  �������P�ɹ��ԡ�Cationic nanoparticles self-assembled from amphiphilic chitosan derivatives containing poly(amidoamine) dendrons and deoxycholic acid as a vector for co-delivery of doxorubicin and gene�����}�� Carbohydrate Polymers (2021��Article 117706) [SCI impact factor: 7.182] �ϰl����Փ�ĵĹ�ͬ��һ���ߞ��ɺɺ��ʿ���������ܲ�ʿ��ͨӍ���ߞ���ɽ��W���ϿƌW�c���̌WԺ������������ԓ�о��õ�������Ȼ�ƌW���𡢏V�|ʡ��Ȼ�ƌW����;ۺ���ͺϲ��ϼ����ܲ��Ͻ��������c����ҵ��Y���c֧�֡�

  Փ��朽�: https://www.sciencedirect.com/science/article/abs/pii/S014486172100093X